Aromatic Acid Metabolites of Escherichia coli K-12 Can Induce the marRAB Operon
Aromatic Acid Metabolites of Escherichia coli K-12 Can Induce the marRAB Operon
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DOI：
10.1128/jb.00371-10
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发表时间：
2010-09-01
影响因子：
3.2
通讯作者：
Rao, Christopher V.
中科院分区：
文献类型：
--
作者：
Chubiz, Lon M.;Rao, Christopher V.
MarR是参与大肠杆菌抗生素耐药性和溶剂胁迫耐受的marRAB操纵子的关键调控因子。我们发现两种代谢中间体，2,3-二羟基苯甲酸酯和邻氨基苯甲酸酯，分别参与肠obactin和色氨酸的生物合成，可以激活marRAB转录。我们还发现，参与泛醌生物合成的第三个中间体，4-羟基苯甲酸酯，在缺乏TolC的情况下激活marRAB转录。然而，在这三种化合物中，只有2,3-二羟基苯甲酸盐直接结合MarR并影响其活性。
MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We also found that a third intermediate involved in ubiquinone biosynthesis, 4-hydroxybenzoate, activates marRAB transcription in the absence of TolC. Of the three, however, only 2,3-dihydroxybenzoate directly binds MarR and affects its activity.