THE FUSOGENIC EFFECT OF SYNTHETIC POLYCATIONS ON NEGATIVELY CHARGED LIPID BILAYERS
THE FUSOGENIC EFFECT OF SYNTHETIC POLYCATIONS ON NEGATIVELY CHARGED LIPID BILAYERS
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DOI：
10.1093/oxfordjournals.jbchem.a121806
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发表时间：
1986-10-01
影响因子：
2.7
通讯作者：
NANGO, M
中科院分区：
文献类型：
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作者：
OKU, N;YAMAGUCHI, N;NANGO, M
研究了合成聚阳离子、聚烯丙胺和聚乙烯亚胺对含有磷脂酰丝氨酸的脂质体的影响以及聚赖氨酸和二价阳离子的影响。聚阳离子的添加引起由磷脂酰丝氨酸和磷脂酰胆碱（摩尔比1:4）组成的超声处理的囊泡的聚集，如通过测量浊度变化所确定的。当聚合物/囊泡的电荷比从 0.23（聚赖氨酸）到 2.5（线性聚乙烯亚胺）时，脂质体的浊度比对照脂质体增加了 10 倍，而在电荷比高达 500 时添加 Ca2+ 或 Mg2+，浊度没有变化。这些聚阳离子还诱导脂质体膜的混合，如脂质中荧光脂质之间的共振能量转移所示。双层，不会引起从钙黄绿素释放确定的剧烈渗透性变化。这些聚阳离子以约 1.0 的电荷比诱导了 50% 的脂质体混合（脂质浓度为 0.05 mM）。然而，最高的共振能量转移是通过添加聚烯丙胺产生的，这导致囊泡之间膜混合的多循环。还研究了聚阳离子诱导的膜混合和磷脂酰丝氨酸脂质体的渗透性变化。在电荷比约为 1.0 时，这些聚合物引起掺入单独囊泡中的荧光脂质的共振能量转移；然而，丙烯丙胺和支链聚乙烯亚胺也会导致脂质体膜的渗透性增加。聚烯丙胺诱导的磷脂酰丝氨酸囊泡的膜混合和渗透性变化取决于聚合物-囊泡电荷比，并且与Ca2+诱导的不同，因为后者在所研究的脂质体浓度下约1mM时引起半最大膜混合或磷脂酰丝氨酸囊泡的渗透性变化。
The effect of synthetic polycations, polyallylamine, and polyethylenimine, on liposomes containing phosphatidylserine was investigated along with that of polylysine and divalent cations. The addition of polycations caused aggregation of sonicated vesicles composed of phosphatidylserine and phosphatidylcholine (molar ratio 1 : 4) as determined by measuring the turbidity changes. Liposomal turbidity increased 10 times compared with that of control liposomes at charge ratios of polymer/vesicle from 0.23 (polylysine) to 2.5 (linear polyethylenimine), while the turbidity was unchanged by the addition of Ca2+ or Mg2+ at charge ratio up to 500. These polycations also induced intermixing of liposomal membranes as indicated by resonance energy transfer between fluorescent lipid incorporated in lipid bilayers, without inducing drastic permeability changes as determined from the calcein release. Fifty percent intermixing of liposomes (0.05 mM as lipid concentration) was induced by these polycations at charge ratios of around 1.0. However, the highest resonance energy transfer was produced by the addition of polyallylamine, which caused multicycles of membrane intermixing between vesicles. Polycation-induced membrane intermixing and permeability changes of phosphatidylserine liposomes were also investigated. At charge ratios of around 1.0, these polymers caused resonance energy transfer of fluorescent lipids incorporated in separate vesicles; however, pollallylamine and branched polyethylenimine also caused permeability increases of liposomal membranes. Membrane intermixing and permeability changes of phosphatidylserine vesicles induced by polyallylamien were dependent on the polymer- vesicle charge ratio, and were different from those induced by Ca2+ since the latter caused half-maximal membrane intermixing or permeability change of phosphatidylserine vesicles at about 1 mM at the liposomal concentrations investigated.