Interaction of human immunodeficiency virus with human sperm in vitro.
Interaction of human immunodeficiency virus with human sperm in vitro.
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人类免疫缺陷病毒与人类精子在体外的相互作用。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
G. Harley
中科院分区：
文献类型：
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作者：
O. Bagasra;M. Freund;J. Weidmann;G. Harley
通过性交在精液中传播人类免疫缺陷病毒（HIV）被认为是该疾病传播的主要病因。为了探索精子在这种传播中的作用，将无细胞HIV-1（IIIB）与人精子共孵育5 h。孵育后，将未附着的HIV-1从细胞上大量洗掉，并通过电子显微镜（EM）和免疫荧光方法确定HIV-1在精子中或精子上的存在和位置。在EM水平上对精子的检查表明，HIV-1附着在精子表面并通过完整的细胞膜进入精子。使用多价抗HIV抗体的免疫荧光研究表明，孵育5小时后，在5-10%的精子头部中存在小的圆形荧光颗粒。对HIV-1受体（CD 4表位）的搜索显示，通过落射荧光显微镜检查发现5-10%的精子上存在OKT 4表位，包括OKT 4A、B、D和F，通过流式细胞术分析发现10-20%的精子上存在OKT 4表位。这些体外数据表明，人类精子可能是通过精液传播HIV的主要细胞成分。
Transmission of human immunodeficiency virus (HIV) in semen by sexual intercourse is believed to be a major etiological factor in the spread of the disease. In order to explore the role of sperm in this transmission, cell-free HIV-1(IIIB) was coincubated with human sperm for 5 h. After incubation, unattached HIV-1 was extensively washed off the cells and the presence and location of HIV-1 in or on the sperm was determined by electron microscopic (EM) and immunofluorescence methods. Examination of sperm at the EM level showed that HIV-1 attaches to the sperm surface and enters the sperm through the intact cell membrane. Immunofluorescence studies, using polyvalent anti-HIV antibodies, demonstrated the presence of small round fluorescent particles in 5-10% of sperm heads after 5 h of incubation. A search for HIV-1 receptors (CD4 epitopes) revealed the presence of OKT4 epitopes, including OKT4A, B, D, and F on 5-10% of sperm by epifluorescence microscopy and on 10-20% of sperm by flow-cytometric analysis. These in vitro data suggest that human sperm may be the primary cellular element in the transmission of HIV by semen.