Matrix stiffness affects spontaneous contraction of cardiomyocytes cultured within a PEGylated fibrinogen biomaterial
Matrix stiffness affects spontaneous contraction of cardiomyocytes cultured within a PEGylated fibrinogen biomaterial
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DOI：
10.1016/j.actbio.2006.09.003
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发表时间：
2007-01-01
影响因子：
9.7
通讯作者：
Seliktar, Dror
中科院分区：
文献类型：
--
作者：
Shapira-Schweitzer, Keren;Seliktar, Dror
心脏细胞移植治疗受损心肌的成功实施依赖于改进的可注射生物材料的开发。基于聚乙二醇（PEG）成分，开发了一种新型生物材料技术，该技术使用具有可控物理化学性质的聚乙二醇化纤维蛋白原。此外，材料的纤维蛋白原骨架赋予细胞固有的生物活性。本研究的目的是通过体外技术探索使用这种生物材料作为心脏组织再生的支架。为此，在PEG化纤维蛋白原构建体中培养新生大鼠心肌细胞。优化细胞接种密度和生物材料组成以获得构建体的最大自发收缩。通过视频图像分析完成收缩模式的定量表征。通过使用PEG和纤维蛋白原的不同组合物改变基质的模量，可以证明材料刚度和组织构建体的收缩幅度之间的负相关性。基质硬度、细胞密度和组织收缩之间的关系也提供了对最终导致结构同步收缩的细胞重塑机制的一些了解。这些发现表明聚乙二醇化纤维蛋白原水凝胶可用作心肌细胞的支架，并提供了通过对基质进行简单的组成修饰来控制细胞重塑的可能性。(C)2006 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Successful implementation of cardiac cell transplantation for treating damaged myocardium relies on the development of improved injectable biomaterials. A novel biomaterial technology using PEGylated fibrinogen has been developed with controllable physicochemical properties based on the poly(ethylene glycol) (PEG) constituent. In addition, the fibrinogen backbone of the material confers inherent bioactivity to cells. The purpose of this investigation was to explore by in vitro techniques the use of this biomaterial as a scaffold for cardiac tissue regeneration. To this end neonatal rat cardiomyocytes were cultivated in PEGylated fibrinogen constructs. The cell-seeding density and biomaterial composition were optimized to obtain maximum spontaneous contraction of the constructs. Quantitative characterization of the contraction pattern was accomplished by video image analysis. It was possible to demonstrate an inverse correlation between the material stiffness and the amplitude of contraction of the tissue constructs by changing the modulus of the matrix using different compositions of PEG and fibrinogen. The relationship between matrix stiffness, cell density and tissue contraction also provided some insight into the mechanism of cellular remodeling that ultimately leads to synchronized contraction of the constructs. These findings indicate that PEGylated fibrinogen hydrogels can be used as a scaffold for cardiomyocytes, and offer the possibility of controlling cellular remodeling via simple compositional modifications to the matrix. (C) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.