Multiple effects of S13 in modulating the strength of intersubunit interactions in the ribosome during translation
Multiple effects of S13 in modulating the strength of intersubunit interactions in the ribosome during translation
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DOI：
10.1016/j.jmb.2005.03.075
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发表时间：
2005-05-27
影响因子：
5.6
通讯作者：
Green, R
中科院分区：
文献类型：
--
作者：
Cukras, AR;Green, R
核糖体蛋白S13位于小亚基的头部区域，在那里它与大核糖体亚基的中心突起相互作用，并通过其延伸的C端与P位点结合的tRNA相互作用。大亚基和小亚基之间的桥接相互作用是动态的，并且被认为是协调翻译周期的分子运动的关键。S13在trna结合位点和易位过程中看到的小亚基头部运动之间提供了直接联系，从而提供了一种可能的信号转导途径。我们已经创建并鉴定了一种缺乏rpsM（S13）的大肠杆菌菌株，并通过对缺乏S13的核糖体的体外检测发现了其亚基关联、起始和易位的显著缺陷。S13中特定桥接元件和tRNA接触元件的靶向突变证明这两个相互作用域在维持翻译保真度方面起着关键作用。因此，这种核糖体蛋白似乎在翻译周期的多个步骤中通过调节亚基相互作用发挥了非必需但重要的作用。2005爱思唯尔有限公司版权所有。
The ribosomal protein S13 is found in the head region of the small subunit, where it interacts with the central protuberance of the large ribosomal subunit and with the P site-bound tRNA through its extended C terminus. The bridging interactions between the large and small subunits are dynamic, and are thought to be critical in orchestrating the molecular motions of the translation cycle. S13 provides a direct link between the tRNA-binding site and the movements in the head of the small subunit seen during translocation, thereby providing a possible pathway of signal transduction. We have created and characterized an rpsM(S13)-deficient strain of Escherichia coli and have found significant defects in subunit association, initiation and translocation through in vitro assays of S13-deficient ribosomes. Targeted mutagenesis of specific bridge and tRNA contact elements in S13 provides evidence that these two interaction domains play critical roles in maintaining the fidelity of translation. This ribosomal protein thus appears to play a non-essential, yet important role by modulating subunit interactions in multiple steps of the translation cycle. 2005 Elsevier Ltd. All rights reserved.