HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities.
HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities.
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DOI：
10.1186/s12879-017-2439-0
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发表时间：
2017-05-16
影响因子：
3.7
通讯作者：
Christodoulou C
中科院分区：
文献类型：
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作者：
Krashias G;Koptides D;Christodoulou C
人乳头瘤病毒（HPV）是最常见的性传播媒介，可导致女性宫颈病变和癌症。目前，塞浦路斯缺乏关于HPV流行率的信息。本研究的目的是评估596名年龄在19-65岁之间的细胞学异常妇女的HPV类型特异性患病率。此外，在一个由348名妇女组成的亚组中，研究了HPV感染与宫颈疾病之间的关系。分别采用PCR和限制性片段长度多态性分析进行HPV检测和分型。总体而言，HPV的患病率为72.8%，并且显示出年龄依赖性，直到45岁患病率才下降（p = 0.0018，χ2）。258名女性（59.4%）感染了高危型HPV，151名（34.8%）感染了低危型HPV，25名（5.8%）感染了未知风险的HPV类型。最常见的高危型HPV为HPV 16（17.7%），其次为HPV 31（12.9%）、HPV 58（7.1%）、HPV 68（4.6%）、HPV 18（4.1%）和HPV 56（3.7%）。在有细胞学检查结果的妇女中，268例（77%）HPV阳性，样本分布如下：188例（74%）有意义不明的非典型鳞状细胞（ASCUS），61例（85.9%）有低度鳞状上皮内病变（L-SIL），19例（82.6%）有高度鳞状上皮内病变（H-SIL）。HPV 16是受L-SIL（19.7%）和H-SIL（15.8%）影响的女性中最常见的类型，HPV 31是受ASCUS影响的女性中最常见的类型（16.5%）。本研究提供了第一个流行病学数据有关的HPV感染率和类型分布在塞浦路斯妇女与细胞学异常。
Human papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19–65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated. HPV detection and typing was carried out using PCR and restriction fragment length polymorphism analysis, respectively. Overall, the HPV prevalence was 72.8%, and it was shown to be age dependent, with a decreasing prevalence until the age of 45 years (p = 0.0018, χ2). Two hundred and fifty-eight women (59.4%) were infected with high-risk HPV, 151 (34.8%) with low-risk HPV, and 25 (5.8%) with HPV types of unknown risk. The most common high-risk HPV type was HPV16 (17.7%), followed by HPV31 (12.9%), HPV58 (7.1%), HPV68 (4.6%), HPV18 (4.1%), and HPV56 (3.7%). Among the women for whom cytology results were available, 268 (77%) were HPV positive, with a sample distribution as follows: 188 (74%) had atypical squamous cells of undetermined significance (ASCUS), 61 (85.9%) had low-grade squamous intraepithelial lesion (L-SIL), and 19 (82.6%) had high-grade squamous intraepithelial lesion (H-SIL). HPV16 was the most common type among women affected by L-SIL (19.7%) and H-SIL (15.8%), with HPV31 being the most common type in women affected by ASCUS (16.5%). The present study provides the first epidemiological data related to HPV prevalence and type distribution in Cypriot women with cytological abnormalities.