Polyacetate and Polycarbonate RNA: Acylating Reagents and Properties
Polyacetate and Polycarbonate RNA: Acylating Reagents and Properties
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DOI：
10.1021/acs.orglett.9b01526
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发表时间：
2019-07-19
期刊：
影响因子：
5.2
通讯作者：
Kool, Eric T.
中科院分区：
文献类型：
--
作者：
Habibian, Maryam;Velema, Willem A.;Kool, Eric T.
RNA 2'-OH基的酰化被广泛应用于RNA的结构定位和功能控制。描述了在rna上安装最小的2-碳酰基的反应，即2'- o -乙酰基和2'- o -碳酸基。通过杂化和热熔实验来评价酰基对双相形成的影响。这两种试剂都可以在较低浓度下通过逆转录酶引物延伸（SHAPE）方法绘制RNA二级结构。
Acylation of RNA at 2'-OH groups is widely applied in mapping RNA structure and recently for controlling RNA function. Reactions are described that install the smallest 2-carbon acyl groups on RNA-namely, 2'-O-acetyl and 2'-O-carbonate groups. Hybridization and thermal melting experiments are performed to assess the effects of the acyl groups on duplex formation. Both reagents can be employed at lower concentrations to map RNA secondary structure by reverse transcriptase primer extension (SHAPE) methods.