Aggregation and biofilm formation of bacteria isolated from domestic drinking water
Aggregation and biofilm formation of bacteria isolated from domestic drinking water
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DOI：
10.2166/ws.2013.115
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Biggs, C. A.
中科院分区：
文献类型：
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作者：
Ramalingam, B.;Sekar, R.;Biggs, C. A.
研究了从饮用水中分离的鞘氨醇杆菌属、嗜客菌属、甲基杆菌属和红球菌属4种细菌的自聚集、共聚集和生物膜形成特性。通过定性（DAPI染色）和半定量（视觉共聚集）方法进行自动和共聚集研究，并且通过结晶紫方法定量由纯培养物或双培养物产生的生物膜。来自半定量视觉聚集方法的结果未显示任何立即的自聚集或共聚集，这通过40，6二脒基-2-苯基吲哚（DAPI）染色方法证实。然而，2小时后，甲基杆菌显示出所有四种分离物中最高的自聚集。甲基杆菌组合在延长的时间段（72小时）显示出双物种之间的最高共聚集。与双物种生物膜产生的生物膜数量相比，纯培养物的生物膜形成可以忽略不计。总体结果表明，从饮用水中分离的细菌的共聚集介导的物种特异性和时间依赖性的相互作用与协同类型的生物膜形成。因此，这项研究的结果是一个有用的步骤，通过确定特定的细菌，促进饮用水分配系统中的聚集或生物膜形成，以协助潜在的控制策略的发展。
The objective of this study was to investigate the autoaggregation, coaggregation and biofilm formation of four bacteria namely Sphingobium, Xenophilus, Methylobacterium and Rhodococcus isolated from drinking water. Auto and coaggregation studies were performed by both qualitative (DAPI staining) and semi-quantitative (visual coaggregation) methods and biofilms produced by either pure or dual-cultures were quantified by crystal violet method. Results from the semi-quantitative visual aggregation method did not show any immediate auto or coaggregation, which was confirmed by the 40,6 diamidino-2-phenylindole (DAPI) staining method. However, after 2 hours, Methylobacterium showed the highest autoaggregation of all four isolates. The Methylobacterium combinations showed highest coaggregation between dual species at extended period of times (72 hours). Biofilm formation by pure cultures was negligible in comparison to the quantity of biofilm produced by dual-species biofilms. The overall results show that coaggregation of bacteria isolated from drinking water was mediated by species-specific and time-dependent interactions with a synergistic type of biofilm formation. The results of this study are therefore a useful step in assisting the development of potential control strategies by identifying specific bacteria that promote aggregation or biofilm formation in drinking water distribution systems.