Nicotiana benthamiana Exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans.
Nicotiana benthamiana Exportin 1 is required for elicitor-induced phytoalexin production, cell death induction, and resistance against potato late blight pathogen Phytophthora infestans.
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本塞姆氏烟草 Exportin 1 是激发子诱导的植物抗毒素产生、细胞死亡诱导以及对马铃薯晚疫病病原体致病疫霉的抗性所必需的。
DOI：
10.1007/s10327-019-00855-9
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kawakita K and Takemoto D.
中科院分区：
文献类型：
--
作者：
Mizuno Y;Imano S;Camagna M;Suzuki T;Tanaka A;Sato I;Chiba S;Kawakita K and Takemoto D.
马铃薯晚疫病是马铃薯最具毁灭性的病原菌之一，其病原菌是卵菌致病疫霉。研究植物对磷的抗性机制。infestans，茄科模式植物本氏烟草（Nicotiana benthamiana）。以前，我们报道了NbNup75，一个核孔复合物蛋白，是抗P所必需的。infestans，表明核孔介导的运输参与诱导防御反应。在这项研究中，我们研究了N。本氏菌输出蛋白1NbXpo 1在抗病中的作用。已知哺乳动物和酵母exportin 1蛋白是核孔介导的蛋白质和RNA输出的调节剂。苯噻菌胺表现出轻微的生长缺陷，并显著降低了对P的抗性。害虫NbXpo 1的基因沉默损害了诱导子INF 1（P. infestans），如植物抗毒素辣椒二醇的产生和细胞死亡的诱导。在NbXpo 1沉默的植物中，辣椒二醇生物合成基因NbEAS和防御相关的MAP激酶NbWIPK显著下调，而编码植物防御素的基因NbPDF和抗微生物蛋白硫堇NbTHI上调。这些结果表明，N. benthamianaXpo1参与激活一组特定的防御相关基因。
The oomycetePhytophthora infestansis the causal agent of potato late blight, one of the most devastating pathogens for potato. To investigate the plant mechanisms for resistance againstP. infestans, the Solanaceae model plantNicotiana benthamianawas employed in this study. Previously, we reported that NbNup75, a nuclear pore complex protein, is required for the resistance againstP. infestans, suggesting that nuclear-pore-mediated transport is involved in the induction of defense responses. In this study, we investigated the role ofN. benthamianaexportin 1NbXpo1in disease resistance. Mammalian and yeast exportin 1 proteins are known as a regulator for nuclear-pore-mediated export of proteins and RNAs.NbXpo1-silencedN. benthamianashowed minor growth defects and significantly decreased resistance toP. infestans. Gene silencing ofNbXpo1compromised defense responses induced by the elicitor INF1 (a secretory protein ofP. infestans), such as production of the phytoalexin capsidiol and induction of cell death. InNbXpo1-silenced plants, the genes for capsidiol biosynthesis,NbEAS, and defense-related MAP kinase,NbWIPK, were significantly downregulated, while genes encoding the plant defensin,NbPDF, and anti-microbial protein thionin,NbTHI, were upregulated. These results indicate thatN. benthamianaXpo1 is involved in activating a specific group of defense-related genes.