Ferrous iron- and sulfur-induced genes in Sulfolobus metallicus
Ferrous iron- and sulfur-induced genes in Sulfolobus metallicus
复制标题
DOI：
10.1128/aem.02589-06
复制
发表时间：
2007-04-01
影响因子：
4.4
通讯作者：
Norris, Paul R.
中科院分区：
文献类型：
--
作者：
Bathe, Stephan;Norris, Paul R.
金属硫化叶菌的基因，似乎是上调的关系，无论是亚铁或硫的生长，确定使用消减杂交的cDNAs。在二价铁上生长过程中上调的基因被发现在一个簇中，并且大多数被预测为编码膜蛋白。定量逆转录-PCR的cDNA显示上调的大多数这些基因在生长过程中的亚铁和黄铁矿相比，在硫的增长。观察到的最高表达水平包括编码与细胞色素c氧化酶亚基和CbsA样细胞色素相似的蛋白质的基因。在此鉴定的基因可能与S. metallicus被称为狐狸基因。对硫化叶菌属（Sulfolobus）的三个种（S. tokodaii，S. acidocaldarius和S. solfataricus），只有S. tokodaii有一簇高度相似的开放阅读框架，只有S.这三个物种的tokodaii也能够氧化亚铁。硫加氧酶-还原酶编码基因被鉴定为硫生长的S. metallicus的蛋白质，预测的蛋白质显示出与先前描述的来自S. tokodaii和Acidianus种。
Genes of Sulfolobus metallicus that appeared to be upregulated in relation to growth on either ferrous iron or sulfur were identified using subtractive hybridization of cDNAs. The genes upregulated during growth on ferrous iron were found in a cluster, and most were predicted to encode membrane proteins. Quantitative reverse transcription-PCR of cDNA showed upregulation of most of these genes during growth on ferrous iron and pyrite compared to results during growth on sulfur. The highest expression levels observed included those for genes encoding proteins with similarities to cytochrome c oxidase subunits and a CbsA-like cytochrome. The genes identified here that may be involved in oxidation of ferrous iron by S. metallicus are termed fox genes. Of three available genomes of Sulfolobus species (S. tokodaii, S. acidocaldarius, and S. solfataricus), only that of S. tokodaii has a cluster of highly similar open reading frames, and only S. tokodaii of these three species was also able to oxidize ferrous iron. A gene encoding sulfur oxygenase-reductase was identified as the source of the dominant transcript in sulfur-grown cells of S. metallicus, with the predicted protein showing high identities to the previously described examples from S. tokodaii and species of Acidianus.