Aberrant methylation of an imprinted gene U2af1-rs1(SP2) caused by its own transgene
Aberrant methylation of an imprinted gene U2af1-rs1(SP2) caused by its own transgene
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DOI：
10.1074/jbc.272.14.9120
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发表时间：
1997-04-04
影响因子：
4.8
通讯作者：
Mukai, T
中科院分区：
文献类型：
--
作者：
Hatada, I;Nabetani, A;Mukai, T
基因组印记是指基因在亲本等位基因上的特异性表达。这一现象背后的确切机制可能涉及DNA甲基化，目前尚不清楚。U2 af 1-rs 1（SP2）是一个印记基因表达的父亲等位基因和甲基化的母亲等位基因。在这里，我们报告了一个人工系统中，内源性印记基因U2 af 1-rs 1的表达和甲基化可以通过与其自身的转基因在睾丸中的相互作用而受到影响。我们认为，有一种机制，在男性配子发生的U2 af 1-rs 1基因是保持未甲基化，在后代中表达，除了在女性配子发生的机制，其中U2 af 1-rs 1基因是甲基化的，并没有在后代中表达。
Genomic imprinting refers to the parental allele-specific expression of genes. The precise mechanism underlying this phenomenon, which may involve DNA methylation, is not yet known. U2af1-rs1(SP2) is an imprinted gene expressed from the paternal allele and is methylated on the maternal allele. Here we report an artificial system in which expression and methylation of the endogenous imprinted gene U2af1-rs1 can be affected by interaction with its own transgene in the testis. We suggest that there is a mechanism in male gametogenesis by which the U2af1-rs1 gene is kept unmethylated to be expressed in the offspring in addition to a mechanism in female gametogenesis by which the U2af1-rs1 gene is methylated and is not expressed in the offspring.