Modulation and mapping of a human plasminogen activator by cell fusion
Modulation and mapping of a human plasminogen activator by cell fusion
复制标题
通过细胞融合调节和绘制人纤溶酶原激活剂
DOI：
10.1016/0092-8674(78)90058-2
复制
发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
E. Reich
中科院分区：
文献类型：
--
作者：
R. Kucherlapati;R. Tepper;A. Granelli‐Piperno;E. Reich
肿瘤细胞、转化细胞和一些正常哺乳动物细胞分泌大量的纤溶酶原激活剂（PA），这是一种精氨酸特异性蛋白酶，可将纤溶酶原转化为纤溶酶。为了研究PA的调控，我们获得了两类鼠-人体细胞杂交体。将小鼠PA+细胞系PG19与C32（人PA+）或人二倍体成纤维细胞（PA-）融合。所有杂交种均分泌PA。在这些杂交体中，用电泳方法区分了人类和小鼠特异性PA。虽然所有的杂交后代都产生了鼠系基因，但许多杂交后代产生了人类基因，有些则没有。所有产生人类PA的杂种都有共同的6号染色体。人类其他染色体的缺失不影响PA的表达，而6号染色体的缺失与人类PA的缺失相关。我们认为6号染色体携带人类PA的结构基因。这些实验也表明，小鼠PA+细胞与人PA-细胞的融合导致了人PA基因的激活。
Neoplastic cells, transformed cells and some normal mammalian cells secrete large amounts of plasminogen activator (PA), an arginine-specific protease which converts plasminogen to plasmin. To study the regulation of PA, we have obtained two classes of mouse-human somatic cell hybrids. PG19, a mouse PA+ cell line, was fused with C32 (human PA+) or human dlploid flbroblasts (PA-). All hybrids secreted PA. Human-and mouse-specific forms of PA were distinguished In these hybrids by electrophoretic methods. While all hybrids produced the murlne PA, many produced the human PA and some did not. All hybrids which produced human PA had chromosome 6 in common. The absence of each of the other human chromosomes did not affect PA expression, while the absence of chromosome 6 correlated with the lack of human PA. We conclude that chromosome 6 carries the structural gene for human PA. These experiments also show that the fusion of mouse PA+ cells with human PA-cells results in the activation of the human PA gene.