Cell migration and polarity on microfabricated gradients of extracellular matrix proteins
Cell migration and polarity on microfabricated gradients of extracellular matrix proteins
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DOI：
10.1021/la0531493
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发表时间：
2006-04-25
期刊：
影响因子：
3.9
通讯作者：
Leckband, DE
中科院分区：
文献类型：
--
作者：
Gunawan, RC;Silvestre, J;Leckband, DE
本文探讨了细胞外基质蛋白的表面密度和浓度分布对大鼠小肠IEC-6细胞迁移的影响。微流体装置用于产生层粘连蛋白的线性固定梯度。本研究调查了陡度和局部浓度对细胞迁移方向的影响。混合装置中进料流中蛋白质的本体浓度决定了装置中层粘连蛋白的梯度分布和局部浓度。使用两组梯度来探索细胞迁移方向性：（i）局部浓度变化相似的梯度，即，相同的梯度陡度，和（ii）具有相似局部浓度的不同梯度。细胞迁移的梯度尖端，独立于本研究中使用的梯度的陡度。在相同的局部浓度下，迁移速率与梯度陡度无关。然而，在高层粘连蛋白密度下，细胞定向性显著降低。
This paper explores the effects of the surface density and concentration profiles of extra cellular matrix proteins on the migration of rat intestinal IEC-6 cells. Microfluidic devices were used to create linear, immobilized gradients of laminin. This study investigated both the impact of the steepness and local concentrations on the directedness of cell migration. The bulk concentrations of proteins in the feed streams in the mixing device determined the gradient profile and the local concentration of laminin in the device. Two sets of gradients were used to explore cell migration directedness: (i) gradients with similar change in local concentration, i.e., the same gradient steepness, and (ii) different gradients with similar local concentrations. Cells migrated tip the gradients, independent of the steepness of the gradients used in this study. At the same local lalninin concentration, the migration rate was independent of the gradient steepness. However, cell directedness decreased significantly at high laminin densities.