Large P body-like RNPs form in C. elegans oocytes in response to arrested ovulation, heat shock, osmotic stress, and anoxia and are regulated by the major sperm protein pathway
Large P body-like RNPs form in C. elegans oocytes in response to arrested ovulation, heat shock, osmotic stress, and anoxia and are regulated by the major sperm protein pathway
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DOI：
10.1016/j.ydbio.2008.02.059
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发表时间：
2008-06-01
影响因子：
2.7
通讯作者：
Schisa, Jennifer A.
中科院分区：
文献类型：
--
作者：
Jud, Molly C.;Czerwinski, Michael J.;Schisa, Jennifer A.
随着秀丽隐杆线虫雌雄同体年龄的增长，精子逐渐耗尽，排卵停滞，卵母细胞在性腺臂中积累。在这些停滞的卵母细胞中形成大的核糖核蛋白（RNP）灶，其中含有RNA结合蛋白和隐蔽的母体mRNA。在交配后65分钟内，RNP病灶分离并进行受精。大多数具有病灶的停滞卵母细胞在受精后产生存活胚胎，表明病灶对卵母细胞功能无害。我们已经确定，灶的形成并不是严格的老化功能，精子蛋白主要途径的体细胞，ceh-18，分支调节卵母细胞灶的形成和解离。我们对卵母细胞RNP灶功能的假设与处理体（P体）和应激颗粒的RNA相关功能相似;在这里，我们显示了P体蛋白DCP-2、CAR-1和CGH-1的三种直系同源物，以及应激颗粒的两种标记物聚腺苷酸结合蛋白（PABP）和TIA-1似乎存在于卵母细胞RNP灶中。我们的研究结果是第一次在体内演示连接组件的P机构和应力颗粒的生殖系的后生动物。此外，我们的数据表明，卵母细胞RNP灶的形成是可诱导的非逮捕卵母细胞热休克，渗透压，或缺氧，类似的诱导应激颗粒在哺乳动物细胞和酵母中的P体。这些数据表明，延迟受精的卵母细胞和受到环境压力的细胞之间在如何调节mRNA和调节翻译方面存在共性。(C)2008年爱思唯尔公司All rights reserved.
As Caenorhabditis elegans hermaphrodites age, sperm become depleted, ovulation arrests, and oocytes accumulate in the gonad arm. Large ribonucleoprotein (RNP) foci form in these arrested oocytes that contain RNA-binding proteins and translationally masked maternal mRNAs. Within 65 min of mating, the RNP foci dissociate and fertilization proceeds. The majority of arrested oocytes with foci result in viable embryos upon fertilization, suggesting that foci are not deleterious to oocyte function. We have determined that foci formation is not strictly a function of aging, and the somatic, ceh-18, branch of the major sperm protein pathway regulates the formation and dissociation of oocyte foci. Our hypothesis for the function of oocyte RNP foci is similar to the RNA-related functions of processing bodies (P bodies) and stress granules; here, we show three orthologs of P body proteins, DCP-2, CAR-1 and CGH-1, and two markers of stress granules, poly (A) binding protein (PABP) and TIA-1, appear to be present in the oocyte RNP foci. Our results are the first in vivo demonstration linking components of P bodies and stress granules in the germ line of a metazoan. Furthermore, our data demonstrate that formation of oocyte RNP foci is inducible in non-arrested oocytes by heat shock, osmotic stress, or anoxia, similar to the induction of stress granules in mammalian cells and P bodies in yeast. These data suggest commonalities between oocytes undergoing delayed fertilization and cells that are stressed environmentally, as to how they modulate mRNAs and regulate translation. (C) 2008 Elsevier Inc. All rights reserved.