Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America.
Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America.
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DOI：
10.7554/elife.65091
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发表时间：
2021-05-04
期刊：
影响因子：
7.7
通讯作者：
Traxler MF
中科院分区：
文献类型：
--
作者：
Pessotti RC;Hansen BL;Reaso JN;Ceja-Navarro JA;El-Hifnawi L;Brodie EL;Traxler MF
一些昆虫形成共生体，其中放线菌通过制造抗菌剂来防御病原体。在这些联系中使用的化学策略的范围，以及这些策略如何与昆虫的生活方式相关，仍然没有得到充分的探索。我们评估了Odontotaenius dissectus物种的亚社会通道甲虫，以及它们的粪便(粪便物质)，这是它们画廊中的重要食物资源，作为昆虫/放线菌系统的模式。通过化学和系统发育分析，我们发现北美东部采集的分离链霉菌含有多个链霉菌谱系和不同的抗菌剂。在FRAS中检测到的代谢产物对一种真菌昆虫病原绿僵菌表现出协同和拮抗抑制作用，而多个链霉菌菌株直接在FRAS中共培养时对该病原菌具有抑制作用。这些发现支持这样一种模型，即分离的O.disunctus的生活方式在他们的兄弟会中容纳了多个链霉菌谱系，导致了丰富的抗菌剂库，可能使他们的画廊免受病原体入侵。
Some insects form symbioses in which actinomycetes provide defense against pathogens by making antimicrobials. The range of chemical strategies employed across these associations, and how these strategies relate to insect lifestyle, remains underexplored. We assessed subsocial passalid beetles of the species Odontotaenius disjunctus, and their frass (fecal material), which is an important food resource within their galleries, as a model insect/actinomycete system. Through chemical and phylogenetic analyses, we found that O. disjunctus frass collected across eastern North America harbored multiple lineages of Streptomyces and diverse antimicrobials. Metabolites detected in frass displayed synergistic and antagonistic inhibition of a fungal entomopathogen, Metarhizium anisopliae, and multiple streptomycete isolates inhibited this pathogen when co-cultivated directly in frass. These findings support a model in which the lifestyle of O. disjunctus accommodates multiple Streptomyces lineages in their frass, resulting in a rich repertoire of antimicrobials that likely insulates their galleries against pathogenic invasion.