A novel mimetic peroxidase catalyst by using magnetite-containing silica nanoparticles as carriers
A novel mimetic peroxidase catalyst by using magnetite-containing silica nanoparticles as carriers
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DOI：
10.1007/s00604-008-0038-x
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发表时间：
2009
影响因子：
5.7
通讯作者：
Xiaolan Chen;Ting-ting Zhao;Jianli Zou
中科院分区：
文献类型：
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作者：
Xiaolan Chen;Ting-ting Zhao;Jianli Zou
通过结合反相微乳液和改进的 Stöber 方法，开发了一种用无定形二氧化硅包覆磁铁矿和模拟酶（氯化血红素）以形成新型模拟过氧化物酶（磁铁矿-氯化血红素/SiO2）的方法。磁性二氧化硅纳米颗粒负载氯化血红素具有长期的温度稳定性和良好的可重复使用性。通过使用外部磁场，它们可以很容易地从反应溶液中分离出来，并直接重新用于下一轮反应。基于磁铁矿-氯化血红素/SiO2对对羟基苯乙酸和H2O2反应的催化作用，研究了其过氧化物酶活性。结果表明，新型模拟酶催化剂的催化活性高于游离氯化血红素。通过对 H2O2 的测定证明了其应用的可能性，检测限为 7.3 nmol L−1H2O2。
A method for coating magnetite and mimetic enzyme (hemin) with amorphous silica to form a novel mimetic peroxidase (magnetite–hemin/SiO2) has been developed by combining reverse microemulsion and the modified Stöber method. The magnetic silica nanoparticle supported hemin has a long-term stability toward temperature and good reusability. They can be easily separated from the reaction solution by using an external magnetic field and reused directly for next round of reaction. The peroxidase activity of the magnetite–hemin/SiO2was studied based on its catalytic effect on the reaction ofp-hydroxyphenylacetic acid and H2O2. The results indicated that the catalytic activity of the new mimetic enzyme catalyst is higher than that of the free hemin. The possibility of its application was proven by the determination of H2O2, with the detection limits of 7.3 nmol L−1H2O2.