Oligonucleotide stabilized silver nanoclusters as fluorescence probe for drug-DNA interaction investigation
Oligonucleotide stabilized silver nanoclusters as fluorescence probe for drug-DNA interaction investigation
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DOI：
10.1016/j.aca.2011.08.043
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发表时间：
2011-11-14
影响因子：
6.2
通讯作者：
Wang, Erkang
中科院分区：
文献类型：
--
作者：
Yuan, Jipei;Guo, Weiwei;Wang, Erkang
本工作成功地将寡核苷酸稳定的银纳米团簇作为新型荧光探针用于药物与DNA的相互作用研究。银纳米团簇对所研究的药物(包括柔红霉素和喹卡林两种插层剂，以及非插层粘合剂双苯甲亚胺H 33258)是灵敏的探针，对所研究的药物的检测下限可达到10(-8)摩尔L-1水平。用非线性拟合法研究了药物与小牛胸腺DNA的相互作用，得到了结合常数和结合部位大小。银纳米团簇作为一种生物相容性材料，在化学特别是生化分析领域具有广阔的应用前景。(C)2011爱思唯尔B.V.保留所有权利。
In this work, oligonucleotide stabilized silver nanoclusters as novel fluorescent probes were successfully utilized for the drug-DNA interaction study. Silver nanoclusters were proved to be sensitive probes for the drugs investigated (including of two kinds of intercalators, daunorubicin and quinacrine, as well as a non-intercalating binder bisBenzimide H 33258), as the detection limits at 10(-8) mol L-1 level of studied drugs can be achieved. The interactions of drugs and calf thymus DNA were investigated using non-linear fit analysis, and the binding constants as well as binding site sizes were obtained. As biocompatible materials, silver nanoclusters are promising in the chemical especially biochemical analysis fields. (C) 2011 Elsevier B.V. All rights reserved.