Increased numbers of both airway basophils and mast cells in sputum after allergen inhalation challenge of atopic asthmatics
Increased numbers of both airway basophils and mast cells in sputum after allergen inhalation challenge of atopic asthmatics
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DOI：
10.1164/ajrccm.161.5.9908090
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发表时间：
2000-05-01
影响因子：
24.7
通讯作者：
O'Byrne, PM
中科院分区：
文献类型：
--
作者：
Gauvreau, GM;Lee, JM;O'Byrne, PM
肥大细胞和嗜碱性粒细胞是参与过敏性炎症的异染色细胞。过敏性哮喘患者的过敏原刺激可增加异染色质细胞水平，这可能反映肥大细胞和/或嗜碱性粒细胞的增加。我们进行了一项研究，利用针对每种细胞的单抗，研究特应性哮喘受试者吸入变应原后，嗜碱性粒细胞和肥大细胞在呼吸道中的募集动力学。在19名受试者中，14人在吸入变应原后同时出现早期和晚期哮喘反应(双反应者[DRS])，而5人只出现早期哮喘反应(早期反应者[ER])。ERS和DRS7小时和24小时的痰嗜酸性粒细胞(P<；0.002)和嗜碱性粒细胞(P<；0.002)数量均显著增加。攻击后7小时和24小时，DRS组大鼠痰中活化的嗜酸性粒细胞(P=0.00002)和肥大细胞数(P=0.009)显著增加，而ERS组则无明显变化(P&gT；0.4)。过敏原诱导的痰嗜碱性粒细胞数显著高于急性期(p<0.01)，且嗜碱性粒细胞与激发后24小时的气道高反应性显著相关(r=0.66，p=0.002)。DRS比ER有更高的变应原诱导的嗜碱性细胞水平，这可能是观察到的AHR的原因。
Mast cells and basophils are metachromatic cells that participate in allergic inflammation. Allergen challenge to the airways of atopic asthmatic individuals increases levels of metachromatic cells, which may reflect an increase in mast cells, basophils, or both. We conducted a study to characterize the kinetics of basophil and mast cell recruitment to the airways of atopic asthmatic subjects after allergen inhalation challenge, using monoclonal antibodies specific for each type of cell. Of 19 subjects, 14 developed both early- and late-phase asthmatic responses (dual responders [DRs]), whereas five developed only early asthmatic responses (early responders [ERs]) after allergen inhalation. There was a significant increase in the number of sputum eosinophils (p < 0.002) and basophils (p < 0.002) at 7 h and 24 h after challenge in both ERs and DRs. There was also a significant increase in the number of activated eosinophils (p = 0.00002) and mast cells (p = 0.009) in sputum at 7 h and 24 h after challenge in DRs, but not in ERs (p > 0.4). DRs had a significantly higher number of allergen-induced sputum basophils than did ERs (p < 0.01), and sputum basophils correlated significantly with airway hyperresponsiveness (AHR) to methacholine at 24 h after challenge (r = 0.66, p = 0.002). DRs tended to have higher allergen-induced basophil levels than did ERs, which may contribute to the observed AHR.