Paper-based microfluidic devices for electrochemical immunofiltration analysis of human chorionic gonadotropin
Paper-based microfluidic devices for electrochemical immunofiltration analysis of human chorionic gonadotropin
复制标题
DOI：
10.1016/j.bios.2017.02.002
复制
发表时间：
2017-06-15
影响因子：
12.6
通讯作者：
Chen, Zhencheng
中科院分区：
文献类型：
--
作者：
Cao, Liangli;Fang, Cheng;Chen, Zhencheng
基于光刻和丝网印刷技术的电化学免疫过滤分析首次被引入微流控纸基分析装置（mu PAD）中。醛功能化丝网印刷电极 (SPE) 的亲水测试区使用捕获抗体 (Ab(1)) 进行生物功能化。利用一抗信号抗体功能化金纳米粒子(GNPs/Ab(2))和碱性磷酸酶偶联二抗(ALP-IgG)建立了灵敏的免疫检测方法。进行微分脉冲伏安法（DPV）来检测电化学响应。对微流体纸基电化学免疫传感器（mu-PEI）进行了优化和表征，用于检测模型分析物人绒毛膜促性腺激素（HCG），线性范围为1.0 mIU mL（-1）至100.0 IU mL（-1），检测限为0.36 mIU mL（-1）。此外，将所提出的mu-PEI用于测试真实人血清中的HCG，并获得了满意的结果。一次性、高效、灵敏且低成本的mu-PEI在开发可应用于医疗保健监测的即时检测（POCT）设备方面表现出了巨大的潜力。
An electrochemical immunofiltration analysis was introduced into microfluidic paper-based analytical devices (mu PADs) for the first time, which was based on photolithography and screen-printing technology. The hydrophilic test zones of the aldehyde-functionalized screen-printed electrodes (SPEs) were biofunctionalized with capture antibodies (Ab(1)). A sensitive immune detection method was developed by using primary signal antibody functionalized gold nanoparticles (GNPs/Ab(2)) and alkaline phosphatase conjugated secondary antibody (ALP-IgG). Differential pulse voltammetry (DPV) was performed to detect the electrochemical response. The microfluidic paper-based electrochemical immunosensor (mu-PEI) was optimized and characterized for the detection of human chorionic gonadotropin (HCG), a model analyte, in a linear range from 1.0 mIU mL(-1) to 100.0 IU mL(-1) with a detection limit of 0.36 mIU mL(-1). Additionally, the proposed mu-PEI was used to test HCG in real human serum and obtained satisfactory results. The disposable, efficient, sensitive and low-cost mu-PEI has exhibited great potential for the development of point-of-care testing (POCT) devices that can be applicated in healthcare monitoring.