TRANSFORMING PROTEIN OF SIMIAN SARCOMA-VIRUS STIMULATES AUTOCRINE GROWTH OF SSV-TRANSFORMED CELLS THROUGH PDGF CELL-SURFACE RECEPTORS
TRANSFORMING PROTEIN OF SIMIAN SARCOMA-VIRUS STIMULATES AUTOCRINE GROWTH OF SSV-TRANSFORMED CELLS THROUGH PDGF CELL-SURFACE RECEPTORS
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DOI：
10.1016/0092-8674(84)90193-4
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
DEUEL, TF
中科院分区：
文献类型：
--
作者：
HUANG, JS;HUANG, SS;DEUEL, TF
猴肉瘤病毒转化的NIH 3 T3（SSV-NIH 3 T3）和SSV-NRK细胞分泌与血小板衍生生长因子（PDGF）在有丝分裂试验中相同的强效生长促进活性。分泌的活性被抗-PDGF抗血清阻断，并与125 I-PDGF竞争受体结合，表明分泌的蛋白是SSV、p28 v-sis或其加工产物的转化蛋白。分泌的p28 v-sis似乎刺激SSV转化细胞的自分泌细胞生长，因为抗PDGF抗血清阻断3 H-胸苷掺入生长的SSV-NIH 3 T3和SSV-NRK细胞。SSV-转化的细胞具有减少的高亲和力125 I-PDGF受体的数量; PDGF/p28 v-sis受体从SSV-NIH 3 T3细胞中纯化，并保留由PDGF刺激的活性蛋白酪氨酸激酶活性。将注射了SSV转化细胞的无胸腺裸小鼠的肿瘤生长速率与分泌的生长因子活性水平进行比较。裸鼠中肿瘤生长的速率与由SSV转化细胞分泌的p28 v-sis的水平直接相关。
Simian sarcoma virus-transformed NIH 3T3 (SSV-NIH 3T3) and SSV-NRK cells secrete a potent growth-promoting activity identical with the platelet-derived growth factor (PDGF) in mitogenic assays. The secreted activity is blocked by anti-PDGF antisera and competes with 125I-PDGF for receptor binding, suggesting that the secreted protein is the transforming protein of SSV, p28v-sis or its processed product. Secreted p28v-sis appears to stimulate autocrine cell growth of SSV-transformed cells because anti-PDGF antisera block 3H-thymidine incorporation into growing SSV-NIH 3T3 and SSV-NRK cells. SSV-transformed cells have reduced numbers of high-affinity 125I-PDGF receptors; PDGF/p28v-sis receptor was purified from SSV-NIH 3T3 cells and retained active protein tyrosine kinase activity stimulated by PDGF. The rate of tumor growth in athymic nude mice injected with SSV-transformed cells was compared with levels of secreted growth factor activity. The rate of tumor growth in nude mice correlated directly with levels of p28v-sis secreted by SSV-transformed cells.