Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera.
Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera.
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来自 Caulerpa lentillifera 的硫酸化多糖的结构特征和 SARS-CoV-2 抑制活性。
DOI：
10.1016/j.carbpol.2021.119006
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发表时间：
2022-03-15
影响因子：
11.2
通讯作者：
Song S
中科院分区：
文献类型：
--
作者：
You Y;Song H;Wang L;Peng H;Sun Y;Ai C;Wen C;Zhu B;Song S
狭叶蕨藻（Caulerpa lentilifera）为绿藻门苔藓藻纲（Bryopsidophyceae）的一种食用海藻，近年来因其养殖规模的扩大和消费量的增加而备受关注。本研究从灵芝中分离得到一种硫酸多糖（CLSP-2）。通过一系列化学和光谱方法鉴定了其化学结构。在这些方法中，温和的酸水解和光催化降解被应用于释放单糖和寡糖片段，其通过HPLC-MSn分析进一步鉴定，提供CLSP-2中的糖序列和硫酸酯取代的信息。结果表明，CLSP-2的主链由→6）-β-Manp-（1→）和C2的硫酸化支链组成，主要由→3）-β-Galp 4S-（1→，→3）-β-Galp 2，4S-（1→）和次要的Xyl组成。病毒中和实验表明，CLSP-2对SARS-CoV-2感染的HeLa细胞有明显的保护作用，其IC 50为48.48 μg/mL。因此，本研究表明CLSP-2是一种有前途的抗SARS-CoV-2药物。
Caulerpa lentillifera (Bryopsidophyceae, Chlorophyta) is an edible seaweed attracting great attention for its expansion of farming scale and increasing consumption in these years. In the present study, a sulfated polysaccharide (CLSP-2) was isolated and separated from C. lentillifera, and its chemical structure was elucidated by a series of chemical and spectroscopic methods. Among these methods, mild acid hydrolysis and photocatalytic degradation were applied to release mono- and oligo-saccharide fragments which were further identified by HPLC-MSn analysis, affording the information of the sugar sequences and the sulfate substitution in CLSP-2. Results indicated that the backbone of CLSP-2 was constructed of →6)-β-Manp-(1→ with sulfated branches at C2, which were comprised of prevalent →3)-β-Galp4S-(1→, →3)-β-Galp2,4S-(1→, and minor Xyl. In addition, the virus neutralization assay revealed that CLSP-2 could effectively protect HeLa cells against SARS-CoV-2 infection with an IC50 of 48.48 μg/mL. Hence, the present study suggests CLSP-2 as a promising agent against SARS-CoV-2.