Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release
Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release
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分层电喷雾PLGA@壳聚糖球包球复合微球用于多药控释
DOI：
10.1093/rb/rbaa009
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发表时间：
2020-08-01
影响因子：
6.7
通讯作者：
Wang, Xiumei
中科院分区：
文献类型：
--
作者：
Liu, Zhu;Ye, Weilong;Wang, Xiumei
摘要 不同药物的顺序给药和控释对于调节细胞行为和组织再生至关重要，这通常需要适当的载体如微球（MS）来控制药物释放。电喷雾已被证明是制备粒径均匀、载药量高的MS的有效技术。在本研究中，我们应用电喷雾简单且分层地制造球包球复合微球，将较小的聚乳酸-乙醇酸共聚物MS（直径~8-10μm）嵌入到较大的壳聚糖MS（直径~250-300μm）中。扫描电子显微镜图像显示高度均匀的质谱，可以通过调整喷嘴直径或电压来精确控制。微球中封装有牛血清白蛋白和醋酸氯己定两种模型药物。荧光标记的异硫氰酸罗丹明荧光素（Rho-FITC）和紫外（UV）分光光度法结果表明，负载的药物在微球中具有良好的分布，并且在体外具有持续、缓慢的释放。此外，远紫外圆二色性和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）结果表明了电喷雾后药物的原始二级结构和分子量。总的来说，我们的研究提出了一种改进的分层电喷雾技术来制备负载两种不同药物的球中球复合MS，可应用于顺序、多模式治疗。
Abstract Sequential administration and controlled release of different drugs are of vital importance for regulating cellular behaviors and tissue regeneration, which usually demands appropriate carriers like microspheres (MS) to control drugs releases. Electrospray has been proven an effective technique to prepare MS with uniform particle size and high drug-loading rate. In this study, we applied electrospray to simply and hierarchically fabricate sphere-in-sphere composite microspheres, with smaller poly(lactic-co-glycolic acid) MS (∼8–10 μm in diameter) embedded in a larger chitosan MS (∼250–300 μm in diameter). The scanning electron microscopy images revealed highly uniform MS that can be accurately controlled by adjusting the nozzle diameter or voltage. Two kinds of model drugs, bovine serum albumin and chlorhexidine acetate, were encapsulated in the microspheres. The fluorescence-labeled rhodamine-fluoresceine isothiocyanate (Rho-FITC) and ultraviolet (UV) spectrophotometry results suggested that loaded drugs got excellent distribution in microspheres, as well as sustained, slow release in vitro. In addition, far-UV circular dichroism and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) results indicated original secondary structure and molecular weight of drugs after electrospraying. Generally speaking, our research proposed a modified hierarchically electrospraying technique to prepare sphere-in-sphere composite MS with two different drugs loaded, which could be applied in sequential, multi-modality therapy.