Effects of DNA methylation on the structure of nucleosomes.
Effects of DNA methylation on the structure of nucleosomes.
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DOI：
10.1021/ja210273w
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发表时间：
2012-01-11
影响因子：
15
通讯作者：
Lee, Tae-Hee
中科院分区：
文献类型：
--
作者：
Lee, Ju Yeon;Lee, Tae-Hee
Nucleosomes are the fundamental packing units of the eukaryotic genome. Understanding the dynamic structure of a nucleosome is a key to the elucidation of genome packaging in eukaryotes, which is tied to the mechanisms of gene regulation. CpG methylation of DNA is an epigenetic modification associated with the inactivation of transcription and the formation of a repressive chromatin structure. Unraveling the changes in the structure of nucleosomes upon CpG methylation is an essential step toward the understanding of the mechanisms of gene repression and silencing by CpG methylation. Here, we report single molecule and ensemble fluorescence studies to show how the structure of a nucleosome is affected by CpG methylation. The results indicate that CpG methylation induces tighter wrapping of DNA around the histone core accompanied by a topology change. These findings suggest that changes in the physical properties of nucleosomes induced upon CpG methylation may contribute directly to the formation of a repressive chromatin structure.
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DOI：
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