Triiodothyronine nuclear receptor in chick embryo: nature and properties of hepatic receptor.
Triiodothyronine nuclear receptor in chick embryo: nature and properties of hepatic receptor.
复制标题
鸡胚中的三碘甲状腺原氨酸核受体：肝受体的性质和特性。
DOI：
10.1210/endo-109-4-1017
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发表时间：
1981
期刊：
影响因子：
4.8
通讯作者：
J. Lehoux
中科院分区：
文献类型：
--
作者：
D. Bellabarba;J. Lehoux
培养17天的鸡胚肝细胞核能与T_3结合。这种结合在37 ℃时最佳，并通过向孵育培养基中加入蛋白水解酶而消除。用0.4 M KCl（pH 8.0）去除约70%的核结合[125 I]T3，并与大分子结合，因为这些提取物中40-60%的放射性被小Sephadex G-50柱排除。甘油密度梯度分析表明，该大分子的沉降系数为4.3S。根据这些数据和Sephadex G-100凝胶过滤的结果，计算出[125 I]T3蛋白复合物的分子量为60，000。两个制备纯化的细胞核和核提取物上进行的结合研究揭示了一类具有受体性质的结合位点。用纯化的细胞核进行的实验中，结合常数为1.04- 1.07 × 109 M ~（-1），而用细胞核提取物进行的实验中，结合常数为2.17 × 109 M ~（-1），结合容量为363 fmol ~（-1）.
Livers uclei from chick embryos of 17 day incubation are capable of binding T3. This binding was optimal at 37 C and was abolished by the addition of proteolytic enzymes to the incubation medium. Approximately 70% of the nuclear bound [125I]T3 was removed with 0.4 M KC1 at pH 8.0 and was bound to a macromolecule, since 40-60% of the radioactivity in these extracts was excluded from small Sephadex G-50 columns. Glycerol density gradient analysis showed that this macromolecule had a sedimentation coefficient of 4.3S. From these data and the results of Sephadex G-100 gel filtration, the molecular weight of the [125I]T3 protein complex was calculated as 60,000. Binding studies performed on two preparations of purified nuclei and on nuclear extracts revealed a single class of binding sites with the properties of a receptor. In the experiments with purified nuclei, the association constant varied between 1.04– 1.07 × 109 M-1 whereas with nuclear extracts it was 2.17 × 109 M-1 The binding capacity was 363 fmol (...