The rat epididymal transcriptome: Comparison of segmental gene expression in the rat and mouse epididymides
The rat epididymal transcriptome: Comparison of segmental gene expression in the rat and mouse epididymides
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DOI：
10.1095/biolreprod.106.057323
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发表时间：
2007-04-01
影响因子：
3.6
通讯作者：
Johnston, Daniel S.
中科院分区：
文献类型：
--
作者：
Jelinsky, Scott A.;Turner, Terry T.;Johnston, Daniel S.
附睾的区域差异对于精子成熟所需的腔内环境的建立至关重要。本研究通过显微解剖鉴定了大鼠附睾的19个形态不同的节段。从每个片段中分离总RNA并进行微阵列分析。对附睾基因表达的片段分析鉴定出超过16000个表达修饰子，而对整个大鼠附睾的RNA谱分析鉴定出大约12000个表达修饰子。筛选一组正常大鼠组织，确定附睾选择性转录本和附睾特异性转录本。此外，超过3500个限定因子被证明存在，并且在任何两个片段之间差异上调或下调超过四倍。本研究补充了我们之前对小鼠附睾基因表达的片段依赖性分析，并允许在数据集之间进行比较分析。MOE430（小鼠）和RAE230_2（大鼠）微阵列上共有492个基因，在两种物种的附睾中表达，并且在每个物种的片段之间差异表达超过4倍。此外，对β防御素基因家族36个成员的深入定量RT-PCR分析显示，在小鼠和大鼠附睾的长度上，β防御素基因家族的表达模式高度保守。这些分析阐明了沿着大鼠附睾长度的整体基因表达模式，并提供了对两种物种附睾中保守和非保守基因表达模式的新评估。此外，这些数据为未来研究介导精子成熟和储存的生物因素提供了强大的资源。
Regional differences along the epididymis are essential for the establishment of the luminal environment required for sperm maturation. In the current study, 19 morphologically distinct segments of the rat epididymis were identified by microdissection. Total RNA was isolated from each segment and subjected to microarray analysis. Segmental analysis of epididymal gene expression identified more than 16000 expressed qualifiers, whereas profiling of RNA from whole rat epididymis identified approximately 12 000 expressed qualifiers. Screening a panel of normal rat tissues identified both epididymal-selective and epididymal-specific transcripts. In addition, more than 3500 qualifiers were shown to be present and differentially upregulated or downregulated by more than fourfold between any two segments. The present study complements our previous segment-dependent analysis of gene expression in the mouse epididymis and allows for comparative analyses between datasets. A total of 492 genes was shown to be present on both the MOE430 (mouse) and RAE230_2 (rat) microarrays, expressed in the epididymis of both species, and differentially expressed by more than fourfold in between segments in each species. Moreover, in-depth quantitative RT-PCR analysis of 36 members of the beta defensin gene family showed highly conserved patterns of expression along the lengths of the mouse and rat epididymides. These analyses elucidate global gene expression patterns along the length of the rat epididymis and provide a novel evaluation of conserved and nonconserved gene expression patterns in the epididymides of the two species. Furthermore, these data provide a powerful resource for the research community for future studies of biological factors that mediate sperm maturation and storage.