The full-length microRNA cluster in the intron of large latency transcript is associated with the virulence of pseudorabies virus.
The full-length microRNA cluster in the intron of large latency transcript is associated with the virulence of pseudorabies virus.
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DOI：
10.1016/j.virol.2018.05.004
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发表时间：
2018-07
期刊：
影响因子：
3.7
通讯作者：
X. Wang;Meifang Zhang;Kai-ping Yan;Qi Tang;Yi-quan Wu;Wen-Bo He;Huan-chun Chen;Zheng-Fei Liu
中科院分区：
文献类型：
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作者：
X. Wang;Meifang Zhang;Kai-ping Yan;Qi Tang;Yi-quan Wu;Wen-Bo He;Huan-chun Chen;Zheng-Fei Liu
伪狂犬病病毒（Pseudorabiesvirus，PRV）属于α疱疹病毒亚科，是Aujeszky病的病原体。大潜伏期转录本（LLT）是PRV中最丰富的转录本，含有一个约4.6kb的microRNA（miRNA）簇编码内含子。为了研究LLT miRNA簇在PRV生命周期中的功能，我们产生了miRNA簇突变病毒（PRV-miR簇）和回复突变病毒。PRV-ΔmiR簇感染细胞的生长动力学分析显示，与野生型和回复突变病毒相比，PRV-ΔmiR簇感染细胞的噬斑显著更小，滴度更低。突变病毒表现出IE 180表达增加和EP 0表达减少。PRV-ΔmiR簇感染小鼠的临床症状表明，该miRNA簇参与了PRV的发病过程。物理参数、病毒散毒试验和SN 50滴度显示miRNA簇增强PRV在猪中的毒力。总的来说，我们的研究结果表明，全长miRNA簇参与PRV的复制和毒力。
Pseudorabies virus (PRV), the etiological pathogen of Aujeszky's disease, belongs to theAlphaherpesvirussubfamily. Large latency transcript (LLT), the most abundant PRV transcript, harbors a ~ 4.6 kb microRNA (miRNA) cluster-encoding intron. To investigate the function of the LLT miRNA cluster during the life cycle of PRV, we generated a miRNA cluster mutation virus (PRV-∆miR cluster) and revertant virus. Analysis of the growth kinetics of PRV-ΔmiR cluster-infected cells revealed significantly smaller plaques and lower titers than the wild-type and revertant viruses. The mutation virus exhibited increased IE180 and decreased EP0 expression. The clinical symptoms observed in mice infected with PRV-ΔmiR cluster revealed that the miRNA cluster is involved in the pathogenesis of PRV. Physical parameters, virus shedding assays, and the SN50titers revealed that the miRNA cluster enhances PRV virulence in pigs. Collectively, our findings suggest that the full-length miRNA cluster is involved in PRV replication and virulence.