Differences in carotenoid composition among Hymenobacter and related strains support a tree-like model of carotenoid evolution
Differences in carotenoid composition among Hymenobacter and related strains support a tree-like model of carotenoid evolution
复制标题
DOI：
10.1128/aem.02306-07
复制
发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Foght, Julia M.
中科院分区：
文献类型：
--
作者：
Klassen, Jonathan L.;Foght, Julia M.
类胡萝卜素是一种结构多样的色素，在生物技术上具有天然着色剂和预防人类疾病的作用。采用高效液相色谱法（HPLC）对19株类胡萝卜素进行了鉴定，其中包括10株来自南极洲维多利亚上冰川（Victoria Upper Glacier）的新分离株。9种化学性质不同的类胡萝卜素，以不同的组合形式存在，用常规的粗分光光度法分析无法分辨，用制备型高效液相色谱法纯化，并用紫外可见吸收光谱和高分辨率质谱法对其进行了表征。所有主要的膜杆菌类胡萝卜素似乎都来自于一个共同的2'-羟基柔性黄质骨架，包括以前未报道的推定己糖基、戊糖基和甲基衍生物。然而，它们的分布与16S rRNA基因的系统发育并不完全相关。因此，类胡萝卜素成分可能是菌株特异性的，并不遵循垂直进化下降的严格均匀模式。
Carotenoids are structurally diverse pigments of biotechnological interest as natural colorants and in the prevention of human disease. The carotenoids present in 19 strains taxonomically related to the poorly described, nonphotosynthetic bacterial genus Hymenobacter, including 10 novel isolates cultivated from Victoria Upper Glacier, Antarctica, were characterized using high-performance liquid chromatography (HPLC). Nine chemically distinct carotenoids, present in various combinations irresolvable by conventional crude spectrophotometric analyses, were purified by preparative HPLC and characterized using UV-visible light absorption spectroscopy and high-resolution mass spectrometry. All major Hymenobacter carotenoids appear to be derived from a common backbone of 2'-hydroxyflexixanthin and include previously unreported presumptive hexosyl, pentosyl, and methyl derivatives. Their distribution does not, however, correlate perfectly with 16S rRNA gene phylogeny. Carotenoid composition, therefore, may be strain specific and does not follow a strictly homogeneous pattern of vertical evolutionary descent.