Expression levels of microRNA-145 and microRNA-10b are associated with metastasis in non-small cell lung cancer
Expression levels of microRNA-145 and microRNA-10b are associated with metastasis in non-small cell lung cancer
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microRNA-145和microRNA-10b的表达水平与非小细胞肺癌的转移相关
DOI：
10.1080/15384047.2016.1139242
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发表时间：
2016-01-01
影响因子：
3.6
通讯作者：
Chen, Jun
中科院分区：
文献类型：
--
作者：
Li, Yongwen;Li, Ying;Chen, Jun
尽管转移仍然是非小细胞肺癌(NSCLC)患者的压倒性死亡原因，但转移的潜在机制仍不清楚。越来越多的证据表明，microRNAs(MiRNAs)在调节肿瘤细胞的侵袭和转移中起着关键作用。比较了在其他肿瘤类型中发挥转移作用的miR-9、miR-10b、miR-145和miR-155在淋巴转移阳性NSCLC和LN阴性NSCLC中的表达。在LN阳性的非小细胞肺癌中，miR-145的表达显著降低(P<0.05)，而miR-10b的表达显著升高(P<0.05)。MiR-145和miR-10b的表达与非小细胞肺癌的淋巴结转移有关(P均<0.001)。此外，miR-10b促进肺癌细胞株A549的迁移和侵袭，而miR-145抑制A549细胞的体外迁移和侵袭能力。提示miR-10b和miR-145可能分别作为癌基因和抑癌基因在非小细胞肺癌转移中起作用。
Although metastasis remains the overwhelming cause of death for patients with non-small cell lung cancer (NSCLC), the underlying mechanisms of metastasis remain unknown. Accumulating evidence suggests that microRNAs (miRNAs) are key players in the regulation of tumor cell invasion and metastasis. Expression of miR-9, miR-10b, miR-145, and miR-155, 4 miRNAs previously shown to play roles in metastasis in other tumor types, was compared in lymph node (LN)-positive NSCLC versus LN-negative NSCLC. Expression of miR-145 was significantly lower in LN-positive NSCLC (P < 0.05), while expression of miR-10b was significantly higher (P < 0.05). Expression of both miR-145 and miR-10b was correlated with lymph node metastasis in NSCLC (both Ps < 0.001). In addition, miR-10b facilitated the migration and invasion of lung cancer cell line A549, while miR-145 suppressed the migration and invasion capacity of A549 in vitro. These results suggest that miR-10b and miR-145 may act as an oncogene or tumor suppressor gene, respectively, in NSCLC metastasis.