Recent Emergence of a Chytrid Fungal Pathogen in California Cascades Frogs (Rana cascadae)
Recent Emergence of a Chytrid Fungal Pathogen in California Cascades Frogs (Rana cascadae)
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DOI：
10.1007/s10393-016-1201-1
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发表时间：
2017-03-01
期刊：
影响因子：
2.5
通讯作者：
Piovia-Scott, Jonah
中科院分区：
文献类型：
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作者：
De Leon, Marina E.;Vredenburg, Vance T.;Piovia-Scott, Jonah
病原真菌 Batrachochytrium dendrobatidis (Bd) 与全球两栖动物数量下降有关，但通常很难辨别 Bd 作为导致已经发生的数量下降的原因的相对重要性。对博物馆标本的回顾性分析使研究人员能够将 Bd 到达的时间与过去两栖动物数量减少的时间联系起来。加利福尼亚州北部的喀斯喀特蛙 (Rana cascadae) 数量急剧减少，但尚不清楚这些减少的开始是否与 Bd 的到来相对应。我们对从博物馆标本中采集的样本进行了定量实时 PCR 分析，以确定北加州喀斯喀特青蛙范围内 Bd 的历史流行情况。我们在 1907 年至 2003 年间采集的 364 个喀斯喀特青蛙标本中，有 13 个（3.5%）检测到了 Bd，其中第一个阳性结果是在 1978 年。贝叶斯分析表明，Bd 在 1973 年至 1978 年间到达该地区，这与 20 世纪 80 年代首次观察到的下降情况非常吻合。
The pathogenic fungus Batrachochytrium dendrobatidis (Bd) has been associated with global amphibian declines, but it is often difficult to discern the relative importance of Bd as a causal agent in declines that have already occurred. Retrospective analyses of museum specimens have allowed researchers to associate the timing of Bd arrival with the timing of past amphibian declines. Cascades frogs (Rana cascadae) have experienced dramatic declines in northern California, but it is not clear whether the onset of these declines corresponds to the arrival of Bd. We used quantitative real-time PCR assays of samples collected from museum specimens to determine historical Bd prevalence in the northern California range of Cascades frogs. We detected Bd in 13 of 364 (3.5%) Cascades frog specimens collected between 1907 and 2003, with the first positive result from 1978. A Bayesian analysis suggested that Bd arrived in the region between 1973 and 1978, which corresponds well with the first observations of declines in the 1980s.