HSP70 homolog functions in cell-to-cell movement of a plant virus
HSP70 homolog functions in cell-to-cell movement of a plant virus
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DOI：
10.1073/pnas.96.26.14771
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发表时间：
1999-12-21
影响因子：
11.1
通讯作者：
Dolja, VV
中科院分区：
文献类型：
--
作者：
Peremyslov, VV;Hagiwara, Y;Dolja, VV
植物冠状病毒编码HSP70(热休克蛋白，70 kDa)家族的同源蛋白。为了便于研究HSP70同源蛋白(HSP70h)在病毒侵染中的作用，对甜菜黄化闭锁病毒(BYV)进行了绿色荧光蛋白的修饰。这种标记病毒能够在细胞之间移动，产生类似于野生型BYV形成的多细胞感染灶。通过替换起始密码子或缺失493个密码子使HSP70h基因失活，导致BYV从细胞到细胞的转移完全停止。在含有HSP70h的BYV变异体中也观察到了相同的运动缺陷表型，这些HSP70h缺乏计算机预测的ATPase结构域或C-末端结构域，或者在假定的ATPase催化位点存在点突变。这些结果表明，HSP70分子伴侣家族中的病毒特异性成员在细胞间转运中发挥功能，并代表另一种类型的植物病毒运动蛋白。
Plant closteroviruses encode a homolog of the HSP70 (heat shock protein, 70 kDa) family of cellular proteins. To facilitate studies of the function of HSP70 homolog (HSP70h) in viral infection, the beet yellows closterovirus (BYV) was modified to express green fluorescent protein. This tagged virus was competent in cell-to-cell movement, producing multicellular infection foci similar to those formed by the wild-type BYV. Inactivation of the HSP70h gene by replacement of the start codon or by deletion of 493 codons resulted in complete arrest of BYV translocation from cell to cell. Identical movement-deficient phenotypes were observed in BYV variants possessing HSP70h that lacked the computer-predicted ATPase domain or the C-terminal domain, or that harbored point mutations in the putative catalytic site of the ATPase. These results demonstrate that the virus-specific member of the HSP70 family of molecular chaperones functions in intercellular translocation and represents an additional type of a plant viral-movement protein.