The single GUV method for revealing the functions of antimicrobial, pore-forming toxin, and cell-penetrating peptides or proteins
The single GUV method for revealing the functions of antimicrobial, pore-forming toxin, and cell-penetrating peptides or proteins
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DOI：
10.1039/c4cp00717d
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发表时间：
2014-01-01
影响因子：
3.3
通讯作者：
Yamazaki, Masahito
中科院分区：
文献类型：
--
作者：
Islam, Md. Zahidul;Alam, Jahangir Md.;Yamazaki, Masahito
我们最近开发了单巨型单层囊泡（GUV）方法来研究生物膜的功能和动力学。单一GUV方法可以提供生物膜中生理现象的基本过程的详细信息，例如它们的速率常数。在这里，我们描述了由抗菌肽（AMP）， magainin 2和成孔毒素（PFT）， lysenin诱导的孔形成过程，通过单一GUV方法揭示。我们得到了几个基本步骤的速率常数，如肽/蛋白诱导的脂质膜孔形成和荧光探针通过孔的膜渗透。还获得了细胞穿透肽（CPP）转运蛋白10 （TP10）进入单个GUV及其诱导的脂质膜孔形成的信息。我们将单GUV方法与其他研究肽/蛋白与脂质膜相互作用的方法（即大单单层囊泡（LUV）悬液法、GUV悬液法和单通道记录法）进行比较，并讨论单GUV方法的优缺点。在此基础上，讨论了单GUV法的优点。
We recently developed the single giant unilamellar vesicle (GUV) method for investigating the functions and dynamics of biomembranes. The single GUV method can provide detailed information on the elementary processes of physiological phenomena in biomembranes, such as their rate constants. Here we describe the process of pore formation induced by the antimicrobial peptide (AMP), magainin 2, and the pore-forming toxin (PFT), lysenin, as revealed by the single GUV method. We obtained the rate constants of several elementary steps, such as peptide/protein-induced pore formation in lipid membranes and the membrane permeation of fluorescent probes through the pores. Information on the entry of the cell-penetrating peptide (CPP), transportan 10 (TP10), into a single GUV and its induced pore formation in lipid membranes was also obtained. We compare the single GUV method with other methods for investigating the interaction of peptides/proteins with lipid membranes (i.e., the large unilamellar vesicle (LUV) suspension method, the GUV suspension method, and single channel recording), and discuss the pros and cons of the single GUV method. On the basis of these data, we discuss the advantages of the single GUV method.