Structure, expression, and functional characterization of the mouse CLP-1 gene
Structure, expression, and functional characterization of the mouse CLP-1 gene
复制标题
DOI：
10.1016/s0378-1119(02)00596-6
复制
发表时间：
2002-06-12
期刊：
影响因子：
3.5
通讯作者：
Siddiqui, MAQ
中科院分区：
文献类型：
--
作者：
Huang, FC;Wagner, M;Siddiqui, MAQ
小鼠CLP-1是一种潜在的心脏转录调节因子，由一个单拷贝基因编码，该基因缺乏内含子，通过交替聚腺苷酸化表达为两个mRNA。这两种mRNA编码相同的41 kDa蛋白质，这是一种新的蛋白质，与称为HIS 1的人类同源物具有85.3%的同源性。小鼠CLP-1在许多组织中以及在早期发育中广泛表达，并且定位于细胞核。CLP-1基因启动子在不同的细胞类型中是有活性的，序列分析显示了许多潜在的心源性转录因子如Nkx 2.5和加塔-4的结合位点，表明在发育中的潜在作用。CLP-1似乎在心脏而不是其他细胞类型中以浓度依赖性方式“压制”心脏MLC-2 v启动子，这表明CLP-1可能与心脏特异性因子相互作用以调节心脏MLC-2 v表达。CLP-1的总体表达模式与LCR-F1和Oct-1相似，这两种广泛表达的转录因子也在细胞特异性基因的转录中发挥特定作用。CLP-1可能是一种能够与细胞特异性转录因子相互作用并增强其作用的转录介质。(C)2002 Elsevier Science B. V.保留所有权利。
Mouse CLP-1, a potential cardiac transcriptional regulatory factor, is encoded by a single copy gene lacking introns that is expressed into two mRNAs via alternative polyadenylation. Both mRNAs encode the same 41 kDa protein, a novel protein that is 85.3% homologous with a human homologue called HIS1. Mouse CLP-1 is widely expressed in a number of tissues as well as in early development and is localized to the nucleus. The CLP-1 gene promoter is active in different cell types and sequence analysis shows a number of potential binding sites for cardiogenic transcription factors such as Nkx2.5 and GATA-4, indicating a potential role in development. CLP-1 appears to 'squelch' the cardiac MLC-2v promoter in a concentration-dependent manner in cardiac but not other cell types, suggesting that CLP-1 may be interacting with a cardiac-specific factor to regulate cardiac MLC-2v expression. The overall expression pattern of CLP-1 is similar to that of LCR-F1 and Oct-1, two widely expressed transcription factors that also play specific roles in the transcription of cell-specific genes. CLP-1 may be a transcriptional mediator capable of interacting with and potentiating cell-specific transcription factors. (C) 2002 Elsevier Science B.V. All rights reserved.