Odontoblastic Exosomes Attenuate Apoptosis in Neighboring Cells
Odontoblastic Exosomes Attenuate Apoptosis in Neighboring Cells
复制标题
成牙本质细胞外泌体减弱邻近细胞的凋亡
DOI：
10.1177/0022034519869580
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发表时间：
2019-08-30
影响因子：
7.6
通讯作者：
Zhang, L.
中科院分区：
文献类型：
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作者：
Wang, H. S.;Yang, F. H.;Zhang, L.
每个成牙细胞与其他成牙细胞紧密相连。它们形成一道防线，能够抵御外界刺激，特别是由龋齿引起的炎症。因此，我们研究了来自成牙细胞的外泌体作为一种细胞间机制，通过这种机制，炎症的成牙细胞免受凋亡的保护。CD63是一种外泌体标记物，在受龋齿影响的牙髓区域高水平表达。我们进行了体外实验，将不同浓度的脂多糖（LPS）应用于成牙细胞样细胞（矿化是在来自顶端乳头的干细胞中诱导的）。高浓度LPS（20µg/mL LPS，严重影响）处理的成牙细胞样细胞表现出外泌体的加速释放，这减弱了低浓度LPS（1µg/mL LPS，轻度影响）处理的成牙细胞样细胞的LPS诱导的细胞凋亡。接下来，我们用氯丙嗪阻断外泌体的摄取，挽救效果消失。根据我们的发现，严重炎症的成牙细胞通过外泌体介导的细胞间信号通路减弱轻度炎症的邻近细胞的凋亡。
Each odontoblast is tightly linked to other odontoblasts. They form a line of defense and are capable of withstanding external stimuli, particularly the inflammation caused by caries. Thus, we investigated exosomes derived from odontoblasts as an intercellular mechanism by which inflamed odontoblasts are protected from apoptosis. CD63, an exosome marker, was expressed at high levels in caries-affected regions of the dental pulp. We conducted an ex vivo experiment by applying different concentrations of lipopolysaccharide (LPS) to the odontoblast-like cells (mineralization was induced in stem cells derived from the apical papilla). Odontoblast-like cells treated with a high concentration of LPS (20 µg/mL LPS, severely affected) exhibited an accelerated release of exosomes, which attenuated the LPS-induced cell apoptosis of odontoblast-like cells treated with a low concentration of LPS (1 µg/mL LPS, mildly affected). Next, we blocked exosome uptake with chlorpromazine, and the rescue effect vanished. Based on our findings, severely inflamed odontoblasts attenuate the apoptosis of mildly inflamed neighboring cells through an exosome-mediated intercellular signaling pathway.