Development of a rapid method for detecting bacterial cells in situ using 16S rRNA-targeted probes.
Development of a rapid method for detecting bacterial cells in situ using 16S rRNA-targeted probes.
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开发一种使用 16S rRNA 靶向探针原位检测细菌细胞的快速方法。
作者：
Braun;D. Sá;Nierzwicki
已经开发出一种使用 16S rRNA 引导的荧光标记寡核苷酸探针快速识别细菌细胞的方法。评估对标记强度影响的参数包括储存时间、固定剂类型、固定时间、甲醇:甲醛处理时间和硼氢化物处理时间。给出了使用多种微生物（革兰氏阳性和革兰氏阴性）的测试结果。使用这种方法，将细胞点样到载玻片上并干燥保存直至杂交。该方法可能特别适用于环境样品，其中包含不同的细胞类型并且在检查之前经常需要储存。
A rapid method for the identification of bacterial cells using 16S rRNA-directed, fluorescently tagged oligonucleotide probes has been developed. The parameters evaluated for their effect on labeling intensity included storage time, type of fixative, time of fixation, treatment time with methanol:formaldehyde and treatment time with borohydride. The results of tests using a variety of microorganisms, both Gram-positive and Gram-negative, are presented. Using this method, cells are spotted onto slides and stored desiccated until hybridized. This method may be especially applicable to environmental samples, which comprise diverse cell types and frequently require storage prior to examination.