Vanadate blocks cyclic AMP-induced Stimulation of sodium and water transport in amphibian epithelia
Vanadate blocks cyclic AMP-induced Stimulation of sodium and water transport in amphibian epithelia
复制标题
钒酸盐阻断环 AMP 诱导的两栖动物上皮细胞钠和水转运的刺激
作者：
R. C. Sousa;A. Grosso
特别是自从 Cantley 等人 1 确定钒酸盐是 (Na+ + K+) ATP 酶的有效抑制剂以及 Balfour 等人 2 报告其在大鼠中具有显着的利尿钠和利尿特性后，人们对钒的生物效应的兴趣日益浓厚。这些发现促使我们研究钒酸盐对两栖动物上皮细胞的影响，两栖动物上皮细胞是一种广泛用于体外研究钠和水的膜转运的模型3,4。我们发现偏钒酸盐对主动钠转运具有类似哇巴因的作用。此外，我们在此报告了该化合物对活细胞的新生物效应：抑制环 AMP 诱导的跨上皮水流刺激。
INTEREST in the biological effects of vanadium has grown particularly since Cantley et al.1 identified vanadate as a potent inhibitor of (Na+ + K+) ATPase and Balfour et al.2 reported its striking natriuretic and diuretic properties in the rat. Such findings prompted us to investigate the effects of vanadate on amphibian epithelia, a model widely used for studying, in vitro, the membrane transport of sodium and water3,4. We found a ouabain-like action of metavanadate on active sodium transport. In addition, we report here a new biological effect of this compound on living cells: the inhibition of cyclic AMP-induced stimulation of transepithelial water flow.