Spontaneous C-cleavage of a mini-intein without its conserved N-terminal motif A
Spontaneous C-cleavage of a mini-intein without its conserved N-terminal motif A
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不含保守 N 末端基序 A 的微型内含肽的自发 C 切割
DOI：
10.1016/j.febslet.2011.06.035
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发表时间：
2011-08-01
期刊：
影响因子：
3.5
通讯作者：
Liu, Xiang-Qin
中科院分区：
文献类型：
--
作者：
Qi, Xingmei;Meng, Qing;Liu, Xiang-Qin
以前，已知断裂内含肽的C-末端片段仅在添加内含肽的N-末端片段时在其C-末端经历可控的C-切割。在这里，我们从Ssp DnaX内含肽构建了一个类似的分裂内含肽，但我们意外地观察到，其C-末端136-aa片段可以进行自发的C-切割，而没有N-末端长达15 aa的片段，并含有保守的内含肽基序A。这种C-切割活性显着降低了保守的内含肽基序B中的保守的Thr残基的突变。这些发现表明，一个强大的内含肽结构的基序A和基序B在蛋白质剪接机制的第三步中的更大的作用的情况下。(C)2011年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Previously, the C-terminal fragment of a split intein was known to undergo controllable C-cleavage at its C-terminus only when the N-terminal fragment of the intein was added. Here we constructed a similar split intein from the Ssp DnaX intein, but we unexpectedly observed that its C-terminal 136-aa fragment could undergo spontaneous C-cleavage without the N-terminal fragment that was up to 15 aa long and contained the conserved intein motif A. This C-cleavage activity was significantly decreased by a mutation of the conserved Thr residue in the conserved intein motif B. These findings suggest a robust intein structure in the absence of motif A and a larger role of motif B in the third step of the protein splicing mechanism. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.