Immunogenicity of 60 novel latency-related antigens of Mycobacterium tuberculosis.
Immunogenicity of 60 novel latency-related antigens of Mycobacterium tuberculosis.
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DOI：
10.3389/fmicb.2014.00517
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发表时间：
2014
影响因子：
5.2
通讯作者：
Domínguez J
中科院分区：
文献类型：
--
作者：
Serra-Vidal MM;Latorre I;Franken KL;Díaz J;de Souza-Galvão ML;Casas I;Maldonado J;Milà C;Solsona J;Jimenez-Fuentes MÁ;Altet N;Lacoma A;Ruiz-Manzano J;Ausina V;Prat C;Ottenhoff TH;Domínguez J
我们在此工作的目的是评估60种分枝杆菌抗原的免疫原性，其中一些以前没有被评估过，特别是一系列新的体内表达的结核分枝杆菌（ve - tb）抗原。我们招募了505名受试者，并将他们分为有潜伏性结核病感染（LTBI）和无潜伏性结核病感染（LTBI）与活动性结核病（TB）患者。用纯化的重组结核分枝杆菌抗原刺激全血过夜和7天后，用ELISA检测干扰素-γ (IFN-γ)水平。几种抗原在统计上可以显著区分不同的个体。我们从所有研究的抗原群中获得了有希望的抗原[休眠生存调节子（DosR调节子）编码抗原；复苏促进因子抗原；IVE-TB抗原;再激活相关抗原]。Rv1733可能是DosR调控子中编码的保守跨膜蛋白，具有很强的免疫原性，能够区分三种结核状态，因此被认为是一种候选生物标志物。Rv2389和Rv2435n分别属于Rpf家族和IVE-TB组抗原，也成为LTBI生物标志物。虽然需要更多的研究来支持我们的发现，但这些抗原的联合使用将是一种有趣的结核病免疫诊断候选方法。
The aim of our work here was to evaluate the immunogenicity of 60 mycobacterial antigens, some of which have not been previously assessed, notably a novel series of in vivo-expressed Mycobacterium tuberculosis (IVE-TB) antigens. We enrolled 505 subjects and separated them in individuals with and without latent tuberculosis infection (LTBI) vs. patients with active tuberculosis (TB). Following an overnight and 7 days stimulation of whole blood with purified recombinant M. tuberculosis antigens, interferon-γ (IFN-γ) levels were determined by ELISA. Several antigens could statistically significantly differentiate the groups of individuals. We obtained promising antigens from all studied antigen groups [dormancy survival regulon (DosR regulon) encoded antigens; resuscitation-promoting factors (Rpf) antigens; IVE-TB antigens; reactivation associated antigens]. Rv1733, which is a probable conserved transmembrane protein encoded in DosR regulon, turned out to be very immunogenic and able to discriminate between the three defined TB status, thus considered a candidate biomarker. Rv2389 and Rv2435n, belonging to Rpf family and IVE-TB group of antigens, respectively, also stood out as LTBI biomarkers. Although more studies are needed to support our findings, the combined use of these antigens would be an interesting approach to TB immunodiagnosis candidates.