In vitro calibration of the equilibrium reactions of the metallochromic indicator dye antipyrylazo III with calcium.
In vitro calibration of the equilibrium reactions of the metallochromic indicator dye antipyrylazo III with calcium.
复制标题
金属显色指示剂染料 antipyrylazo III 与钙的平衡反应的体外校准。
DOI：
10.1016/s0006-3495(87)83360-x
复制
发表时间：
1987
影响因子：
3.4
通讯作者：
Baylor,SM
中科院分区：
文献类型：
--
作者：
Hollingworth,S;Aldrich,RW;Baylor,SM
用分光光度法研究了金属色指示剂Antipyrylazo III与钙在生理离子强度下的平衡反应。在不同的总染料浓度和钙浓度下，测定了染料吸光度随波长的变化。吸光度分析表明，在pH值为6.9时，至少形成三种钙染料配合物，其化学计量比为1:1、1:2，可能为2:2。测定了解离常数和染料消光系数对1:2配合物形成的影响。1:2配合物是安替吡唑研究细胞质钙瞬态时形成的主要配合物。
The equilibrium reactions of the metallochromic indicator dye Antipyrylazo III with calcium at physiological ionic strength have been investigated spectrophotometrically. Dye absorbance as a function of wavelength was measured at various total dye and calcium concentrations. Analysis of the absorbance spectra indicated that at pH 6.9 at least three calcium:dye complexes form, with 1:1, 1:2, and possibly 2:2 stoichiometries. The dissociation constant and the changes in dye extinction coefficients on formation of the 1:2 complex, the main complex which forms when Antipyrylazo III is used to study cytoplasmic calcium transients, have been characterized.