Intramembranous bone tissue response to biodegradable octacalcium phosphate implant
Intramembranous bone tissue response to biodegradable octacalcium phosphate implant
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DOI：
10.1016/j.actbio.2008.12.008
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发表时间：
2009-06-01
影响因子：
9.7
通讯作者：
Suzuki, O.
中科院分区：
文献类型：
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作者：
Kikawa, T.;Kashimoto, O.;Suzuki, O.
先前的研究表明，当植入动物骨缺损时，合成磷酸八钙 (OCP) 比不可生物降解的羟基磷灰石 (HA)（包括烧结 HA 陶瓷）更能增强骨形成，并且其自身的破骨细胞生物降解作用更强。本研究旨在研究与非烧结化学计量 HA 相比，颗粒形式的合成 OCP 植入小鼠颅骨骨膜下区域时是否具有可生物降解的特性，特别是在植入后相对较短的时间内。 CCP晶体呈现板状形态，而HA晶体具有球状结构。两种晶体均具有大于 75% 的大孔体积，颗粒内有微孔。直到植入后 35 天，通过钙和磷元素分析，HA 中才可辨别新形成骨的直接结合。然而，组织形态分析表明，在长达 21 天的时间内，OCP 表面的碱性磷酸酶活性比 HA 更高，有利于骨形成。抗酒石酸盐酸性磷酸酶阳性破骨细胞样细胞攻击的表面明显大于HA。 OCP 被封装并被新骨取代，植入期延长至 180 天。结果表明，小鼠颅盖骨形成的增强可能与 OCP 的可生物降解性质有关，并且 OCP 可用于增加膜内骨量。 (c) 2008 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Previous studies showed that synthetic octacalcium phosphate (OCP) enhances bone formation coupled with its own osteoclastic biodegradation more than non-biodegradable hydroxyapatite (HA), including sintered HA ceramic, when implanted in animal bone defects. The present study was designed to investigate whether synthetic OCP in granule form has biodegradable characteristics when implanted in the subperiosteal area of mouse calvaria in comparison with non-sintered stoichiometric HA, especially in relatively short periods after implantation. CCP crystals exhibited plate-like morphology, whereas HA crystals had a sphere-like structure. Both crystals had large pore volumes >75% in total, with micropores within the granules. Direct bonding of newly formed bone was discernible in HA until 35 days after implantation by element analysis for calcium and phosphorus. However, histomorphometric analysis demonstrated that bone formation was facilitated on OCP surfaces with greater alkaline phosphatase activity than on HA up to 21 days. The surfaces attacked by tartrate-resistant acid phosphatase positive osteoclast-like cells were significantly greater than those of HA. OCP became encapsulated and replaced with new bone with prolonged implantation periods up to 180 days. The results suggest that the enhanced bone formation in mouse calvaria could be associated with the biodegradable nature of OCP, and that OCP could be used in augmenting intramembranous bone volume. (c) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.