Chronic treatment of aged mice with L-deprenyl produces marked striatal MAO-B inhibition but no beneficial effects on survival, motor performance, or nigral lipofuscin accumulation.
Chronic treatment of aged mice with L-deprenyl produces marked striatal MAO-B inhibition but no beneficial effects on survival, motor performance, or nigral lipofuscin accumulation.
复制标题
用 L-丙炔苯丙胺长期治疗老年小鼠会产生明显的纹状体 MAO-B 抑制作用，但对生存、运动性能或黑质脂褐质积累没有有益影响。
DOI：
10.1016/0197-4580(93)90101-g
复制
发表时间：
1993
影响因子：
4.2
通讯作者：
Gupta,M
中科院分区：
文献类型：
--
作者：
Ingram,DK;Wiener,HL;Chachich,ME;Long,JM;Hengemihle,J;Gupta,M
从18月龄开始，在雄性C57 BL/6J小鼠的饮用水中提供I-丙炔苯丙胺（每天0、0.5 mg/kg或1.0 mg/kg）。在给药前和6个月后24月龄时进行一系列运动试验，包括旷场、钢丝绳、旋转棒、斜屏、转轮和旋转鼓试验。在27月龄时再次对小鼠的子样本进行重新测试。一组未经处理的9个月大的小鼠作为年轻的对照组。丙炔苯丙胺治疗6个月后，纹状体单胺氧化酶-B活性降低了60%，但对纹状体儿茶酚胺水平没有显着影响。未观察到丙炔苯丙胺治疗对小鼠体重、液体摄入量或存活率的显著影响。除27月龄时的转鼓性能外，长期丙炔苯丙胺给药也未影响任何试验中的运动性能，1.0 mg/kg给药组的转鼓性能显著优于对照组。未观察到年龄或丙炔苯丙胺对黑质细胞计数的影响。然而，黑质细胞含有脂褐素随着年龄的增长而增加，但这个神经组织化学参数也不受丙炔苯丙胺治疗。
Male C57BL/6J mice were provided I-deprenyl (at 0, 0.5 mg/kg or 1.0 mg/kg per day) in their drinking water beginning at 18 months of age. A battery of motor tests, including open-field, tightrope, rotorod, inclined screen, runwheel, and rotodrum tests, was administered before treatment and then 6 months later at 24 months of age. A subsample of mice was retested again at 27 months of age. An untreated group of 9-month-old mice served as young controls. Deprenyl treatment reduced striatal MAO-B activity by up to 60% after 6 months on treatment but had no significant effects on striatal catecholamine levels. No significant effects of deprenyl treatment were observed on body weight, fluid intake, or survival of the mice. Chronic deprenyl treatment also did not affect motor performance in any test, except rotodrum performance at 27 months of age, which was significantly better in the 1.0 mg/kg group treated group compared to controls. No age or deprenyl effects were observed with respect to cell counts in the substantia nigra. However, nigral cells containing lipofuscin increased with age, but this neurohistochemical parameter was also unaffected by deprenyl treatment.