Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis).
Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis).
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指猴 (Daubentonia madagascariensis) 的染色体特征和 rDNA 基因座定位。
DOI：
10.1159/000132952
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Miller,DA
中科院分区：
文献类型：
--
作者：
Tagle,DA;Goodman,M;Miller,DA
本文描述了原猴灵长类(Daubentonia Madagascariensis)的核型。报告了各种染色方法(Q-、R-、G-和C-显带、地塞米松A/DAPI和甲基绿/DAPI)的染色结果。用5-甲基胞嘧啶抗体显示甲基化部位。用限制性内切酶消化固定的中期染色体产生G显带。测试的其他限制性内切酶都没有产生清晰的G-或C-带型。核仁组成区(NORs)的银染显示rDNA位点位于最小的一对顶着丝粒染色体13P和14P的短臂上。
The karyotype of a prosimian primate, the aye-aye (Daubentonia madagascariensis) is described. Results from a variety of staining methods (Q-, R-, G-, and C-banding, distamycin A/DAPI and methyl-green/DAPI) are reported. Sites of methyla-tion were visualized using antibodies against 5-methylcytosine. Digestion of aye-aye fixed metaphase chromosomes with the restriction endonucleaseHaelllproduced G-banding. No other restriction enzymes tested produced clear G- or C-banding patterns. Ag-staining of the nucleolar organizer regions (NORs) revealed the location of rDNA sites on the short arms of the smallest pairs of acrocentric chromosomes, 13p and 14p.