Identification, evolution, and regulation of expression of guinea pig trappin with an unusually long transglutaminase substrate domain
Identification, evolution, and regulation of expression of guinea pig trappin with an unusually long transglutaminase substrate domain
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DOI：
10.1074/jbc.m501678200
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发表时间：
2005-05-27
影响因子：
4.8
通讯作者：
Hirose, S
中科院分区：
文献类型：
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作者：
Furutani, Y;Kato, A;Hirose, S
在人类、牛、河马和猪科动物中发现有trappin（一种蛋白质），但在大鼠和小鼠中没有。为阐明trappin基因的进化及其产物的功能意义，我们在豚鼠中分离出了trappin基因，豚鼠属于一个不同于大鼠和小鼠的啮齿动物家族。已证实豚鼠trappin编码的结构域与trappin相同，由一个信号序列、一个超大的转谷氨酰胺酶底物结构域和一个乳清酸性蛋白基序组成。Northern杂交分析、原位杂交组织化学以及免疫组织化学表明，豚鼠trappin仅在精囊的分泌上皮中表达，且其表达依赖雄激素。我们证实豚鼠trappin可被前列腺转谷氨酰胺酶交联，并且源自豚鼠trappin的乳清酸性蛋白基序对白血球弹性蛋白酶具有抑制活性。基因组序列分析表明，豚鼠trappin属于REST（快速进化的精囊转录）基因家族。
Trappins are found in human, bovine, hippopotamus, and members of the pig family, but not in rat and mouse. To clarify the evolution of the trappin genes and the functional significance of their products, we isolated the trappin gene in guinea pig, a species belonging to a rodent family distinct from rat and mouse. Guinea pig trappin was confirmed to encode the same domain structure as trappin, consisting of a signal sequence, an extra large transglutaminase substrate domain, and a whey acidic protein motif. Northern blot analysis and in situ hybridization histochemistry as well as immunohistochemistry demonstrated that guinea pig trappin is expressed solely in the secretory epithelium of the seminal vesicle and that its expression is androgen-dependent. We confirmed that guinea pig trappin is crosslinked by prostate transglutaminase and that the whey acidic protein motif derived from guinea pig trappin has an inhibitory activity against leukocyte elastase. Genome sequence analysis showed that guinea pig trappin belongs to the family of REST (rapidly evolving seminal vesicle transcribed) genes.