Dendritic cells and resting B cells form clusters in vitro and in vivo: T cell independence, partial LFA-1 dependence, and regulation by cross-linking surface molecules.
Dendritic cells and resting B cells form clusters in vitro and in vivo: T cell independence, partial LFA-1 dependence, and regulation by cross-linking surface molecules.
复制标题
树突状细胞和静息 B 细胞在体外和体内形成簇：T 细胞独立性、部分 LFA-1 依赖性以及交联表面分子的调节。
作者：
Natalyia Kushnir;Liming Liu;G. Macpherson
Ab应答的起始需要树突状细胞（DC）、T细胞和T细胞区域中的B细胞之间的相互作用。我们证明了大鼠DC和B细胞在体外和体内形成不依赖于T细胞的簇。体外簇在1小时内形成，并在24至48小时内解离。成簇仅限于静息B细胞，是能量、细胞骨架和蛋白激酶C依赖性的，并且被抗LFA-1但不被抗ICAM-1 mAb抑制。脾和淋巴结B细胞比淋巴或血液中的细胞聚集更强，表明在跨内皮迁移过程中，CD 4+细胞上调。骨髓B细胞不形成簇。来自脾和淋巴结的DC表现出最多的聚集，淋巴源性DC居中，来自固有层、派伊尔集合淋巴结和来自骨髓的DC形成最少的聚集。通过交联DC或B细胞上的II类MHC（全mAb或F（ab '）2）或DC上的Thy-1而不是I类MHC、CD 45或CD 44来刺激成簇。mAb的刺激是能量、细胞骨架和蛋白激酶C依赖性的，但不受抗LFA-1 mAb的抑制，表明涉及其他未鉴定的粘附分子。我们认为，DC和B细胞之间的相互作用将定期发生在B细胞再循环。通过特异性T细胞交联DC上的MHC II类肽分子将增加结合亲合力，导致Ag特异性B细胞在DC上保留足够长的时间以使B细胞处理Ag，从而促进T和B细胞之间的同源相互作用。
Initiation of an Ab response requires interaction between dendritic cells (DC), T cells, and B cells in a T cell area. We demonstrate that rat DC and B cells form T cell-independent clusters in vitro and in vivo. In vitro clusters form within 1 h and dissociate within 24 to 48 h. Clustering is restricted to resting B cells, is energy, cytoskeleton, and protein kinase C dependent, and is inhibited by anti-LFA-1 but not anti-ICAM-1 mAbs. Spleen and lymph node B cells cluster more strongly than those from lymph or blood, suggesting up-regulation of adhesiveness during transendothelial migration. Bone marrow B cells do not form clusters. DC from spleen and lymph nodes show the most clustering, lymph-borne DC are intermediate, and DC from lamina propria, Peyer's patches, and those grown from bone marrow form the fewest clusters. Clustering is stimulated by cross-linking MHC class II (whole mAb or F(ab')2) on DC or B cells or Thy-1 on DC, but not MHC class I, CD45, or CD44. Stimulation by mAb is energy, cytoskeletal, and protein kinase C dependent, but is not inhibited by anti-LFA-1 mAbs, suggesting involvement of other, unidentified adhesion molecules. We suggest that interactions between DC and B cells will occur regularly during B cell recirculation. Cross-linking of MHC class II-peptide molecules on DC by specific T cells would increase binding avidity, causing retention of Ag-specific B cells on DC long enough for the B cells to process Ag, thereby facilitating cognate interactions between T and B cells.
通过表面 Ig 受体刺激 B 淋巴细胞诱导 LFA-1 和 ICAM-1 依赖性粘附。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dang,LH;Rock,KL
通讯作者：
Rock,KL
鉴定 I 区限制性抗原呈递至 T 淋巴细胞所需的巨噬细胞抗原加工事件。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ziegler,K;Unanue,ER
通讯作者：
Unanue,ER