The correlation of angiogenesis with metastasis in primary cutaneous melanoma:: a comparative analysis of microvessel density, expression of vascular endothelial growth factor and basic fibroblastic growth factor
The correlation of angiogenesis with metastasis in primary cutaneous melanoma:: a comparative analysis of microvessel density, expression of vascular endothelial growth factor and basic fibroblastic growth factor
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DOI：
10.1080/00313020600557565
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发表时间：
2006-04-01
期刊：
影响因子：
4.5
通讯作者：
Kaner, G
中科院分区：
文献类型：
--
作者：
Demirkesen, C;Büyükpinarbasili, N;Kaner, G
目的：探讨原发性皮肤黑色素瘤（PCMM）的血管生成与转移之间是否存在相关性。我们研究了22例原发性PCMM伴转移（转移组）和28例无转移24个月以上（非转移组）的微血管密度和血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）的表达。用CD 31/PECAM-1抗体染色微血管并计数。我们评估了VEGF在肿瘤细胞、浸润肿瘤的淋巴细胞（TIL）和肿瘤周围淋巴细胞中的表达比例，以及bFGF在肿瘤细胞胞浆、细胞核和瘤内和瘤周血管中的表达比例。在转移组中检测到微血管密度增加（15 - 33 [ 24.09 +/- 5.55] vs. 2 - 24 [12.96 +/- 6.02]）。此外，肿瘤细胞和瘤周淋巴细胞中VEGF表达增强（卡方检验p=0.038和p=0.018），瘤周血管中bFGF表达增强（卡方检验p=0.013）与PCMM中同时存在黑色素瘤转移相关。微血管密度与瘤周血管中bFGF的表达（rs=0.53，p=0.049）和肿瘤细胞中VEGF的表达（rs=0.37，p=0.019）相关。
Aim: To establish whether there is a correlation between angiogenesis and metastasis in primary cutaneous melanoma (PCMM).Methods: We studied the microvessel density and the expression of vascular endothelial growth factor ( VEGF) and basic fibroblastic growth factor ( bFGF) in 22 cases of PCMM with metastasis at presentation ( metastatic group) and 28 cases of PCMM without metastasis for 24 months or more (non-metastatic group). Microvessels were stained with CD31/PECAM-1 antibody and counted. We assessed the proportion of VEGF expression in tumour cells, lymphocytes infiltrating the tumour (TIL) and lymphocytes at the periphery of the tumour, as well as the proportion of bFGF expression in tumour cell cytoplasms, nuclei and intra-and peritumoral vessels.Results: An increased microvessel density was detected in the metastatic group ( 15 - 33 [ 24.09 +/- 5.55] versus 2 - 24 [12.96 +/- 6.02]). Moreover, enhanced expression of VEGF in tumour cells and peritumoral lymphocytes (Chi-square p=0.038 and p=0.018) and bFGF in peritumoral vessels (chi(2) p=0.013) correlated with the simultaneous presence of melanoma metastasis in PCMM. Furthermore, microvessel density was correlated with the expression of bFGF in peritumoral vessels (rs=0.53, p=0.049) and VEGF in tumour cells ( rs=0.37, p=0.019).Conclusion: Microvessel density as well as the expression of both VEGF and bFGF might be informative concerning the progression of melanoma.