Urinary sex steroid excretion levels during a soy intervention among young girls: A pilot study
Urinary sex steroid excretion levels during a soy intervention among young girls: A pilot study
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DOI：
10.1207/s15327914nc5201_3
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Franke, AA
中科院分区：
文献类型：
--
作者：
Maskarinec, G;Morimoto, Y;Franke, AA
在生命早期摄入大豆可能会通过改变性激素代谢来预防晚年的乳腺癌。在一项为期8周的试验中，我们评估了20名8-14岁笼子女孩尿性类固醇排泄量的可行性。这些女孩每天食用一份大豆，每周收集过夜尿样，报告乳房和阴毛发育的坦纳阶段。性类固醇排泄量用气相色谱-质谱仪一式两份测量，并调整尿肌酐。雌酮、雌二醇、雌三醇、睾酮、孕二醇的变异系数分别为11.4%、10.4%、8.4%、12.8%和4.6%。统计分析*包括t检验。斯皮尔曼的相关性和方差分析。17名女孩完成了这项研究，并表现出对干预策略的良好遵从性。在研究期间，我们观察到总雄激素(0.11mUg/毫克肌酐)和总雌激素(0.001微克/毫克肌酐)没有显著增加，孕二醇(-0.03mgg/毫克肌酐)也没有显著下降。阴毛发育的坦纳阶段越高，雌激素量增加9倍，雄激素排出量增加4倍，孕二醇排泄量增加2倍(P=0.001，P=0.047)。根据乳房发育和月经初潮状况进行分层后，观察到类似的差异。性类固醇水平与青春期发育的关联支持了性类固醇测量的有效性。
Soy intake early in life may protect against breast cancer later in life, possibly by altering sex hormone metabolism. We evaluated the feasibility of assessing urinary sex steroid excretion among 20 young girls caged 8-14 yr in an 8-wk trial. The girls consumed one daily soy serving, collected weekly overnight urine samples, and reported Tanner stages for breast and pubic hair development.,Sex steroid excretion was measured in duplicate by gas chromatography-mass spectrometry and adjusted for urinary creatinine. The respective coefficients of variation for estrone, estradiol, estriol, testosterone, pregnanediol were 11.4%, 10.4%, 8.4%, 12.8%, and 4.6%. The statistical analysis* included t-tests,. Spearman's correlations, and analysis of variance. Seventeen girls completed the study and showed good compliance with the intervention strategy. We observed nonsignificant increases in total androgens (0.11 mu g/mg creatinine) and total estrogens (0.001 mu g/mg creatinine) and a nonsignificant decrease in pregnanediol (-0.03 mu g/mg creatinine) during the study period. Higher Tanner stagesfor pubic hair development were associated with ninefold higher estrogen, fourfold higher androgen, and twofold higher pregnanediol excretions (P = 0.01, P.< 0.001, and P = 0.047, respectively). Similar differences were observed after stratification by, breast development and menarcheal status. The association of sex steroid levels with pubertal development supports the validity of the sex steroid measurements.