Unexpected expression of carbonic anhydrase I and selenium-binding protein as the only major non-heme proteins in erythrocytes of the subterranean mole rat (Spalax ehrenbergi).
Unexpected expression of carbonic anhydrase I and selenium-binding protein as the only major non-heme proteins in erythrocytes of the subterranean mole rat (Spalax ehrenbergi).
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碳酸酐酶 I 和硒结合蛋白作为地下鼹鼠 (Spalax ehrenbergi) 红细胞中唯一主要非血红素蛋白的意外表达。
DOI：
10.1016/s0014-5793(98)00690-5
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Tashian,RE
中科院分区：
文献类型：
--
作者：
Yang,H;Nevo,E;Tashian,RE
从来自以色列的超级种埃伦贝格种地下鼹鼠的红细胞中分离出非血红素蛋白，发现有两个主要峰。在序列分析中，较大的峰对应于先前从小鼠和人的肝脏中鉴定的56 kDa的硒结合蛋白(SeBP)，第二个峰对应于低活性碳酸酐酶(CA)同工酶CA I。到目前为止，没有证据表明在所有被测试的羊水动物的红细胞中都发现了高活性的CA II同工酶。因此，鼹鼠似乎是第一个同时表达SeBP和低活性CA I同工酶的哺乳动物物种，作为其红细胞中的主要非血红素蛋白。高活性CAⅡ同工酶的缺失可能有利于鼹鼠适应其地下洞穴的低O2和高CO2环境。56 kDa的SeBP也可能在红细胞的生理中发挥重要的适应性作用。
Chromatographic separation of the non-heme proteins from the erythrocytes of the subterranean mole rat belonging to the superspecies Spalax ehrenbergi from Israel revealed two major peaks. On sequence analyses, the larger peak corresponded to a 56 kDa selenium-binding protein (SeBP) previously characterized from mouse and human liver, and the second peak to the low-activity carbonic anhydrase (CA) isozyme, CA I. There was no evidence of the high-activity CA II isozyme normally found in the red cells of all amniotes tested to date. Thus, the mole rat appears to be the first mammalian species to express both a SeBP and the low-activity CA I isozyme, as the major non-heme proteins in its red blood cells. It is possible that the absence of the high-activity CA II isozyme may be advantageous to the mole rat in adapting to the low O2and high CO2environment of its underground burrows. It is also likely that the 56 kDa SeBP may play an important adaptive role in the physiology of the red cell.