Differential genotoxicity of chemical properties and particle size of rare metal and metal oxide nanoparticles
Differential genotoxicity of chemical properties and particle size of rare metal and metal oxide nanoparticles
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DOI：
10.1002/jat.1719
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Ishihara, Yoko
中科院分区：
文献类型：
--
作者：
Hasegawa, Go;Shimonaka, Motoyuki;Ishihara, Yoko
稀有金属化合物的纳米颗粒用于各种产品。然而，它们的致癌性和遗传毒性尚未得到充分的评价。研究了四种具有明确粒径的稀有金属：氧化铟（In2O3）、氧化镝（Dy2O3）、氧化钨（WO3）和钼（Mo）的致瘤和促瘤潜能。利用5株细菌的Ames试验研究了这些稀有金属的诱变性，并用v- ha -ras转染BALB/c 3T3细胞（Bhas 42细胞）的细胞转化试验研究了这些稀有金属的可转化性。纳米级Dy2O3在代谢激活和不代谢激活条件下均表现出较强的诱变作用，而微米级Dy2O3在两种细菌中表现出较弱的诱变作用。纳米级和微级Dy2O3诱导Bhas 42细胞的转化菌落呈剂量依赖性，但转化病灶的数量在纳米级和微级颗粒之间没有差异。纳米尺寸的In2O3和WO3分别对TA1537和TA98有正向诱变作用，而微米尺寸的金属氧化物颗粒对试验菌株没有诱变作用。纳米尺寸和微尺寸的In2O3均表现出相似的可变形性。而纳米级和微级WO3均不表现出可变形性。纳米级和微米级Mo颗粒均不具有诱变性和可转化性。这些结果表明，稀有金属的致突变性取决于它们的颗粒大小，尽管可变形性取决于它们的化学成分而不是它们的颗粒大小。版权所有：John Wiley & Sons， Ltd。
Nanoparticles of rare metal compounds are used in various products. However, their carcinogenicity and genotoxicity have not been sufficiently evaluated. The tumor-initiating and -promoting potentials of four rare metals, indium oxide (In2O3), dysprosium oxide (Dy2O3), tungsten oxide (WO3) and molybdenum (Mo), with a well-defined particle diameter were evaluated. The mutagenicity of these rare metals was investigated by Ames test using five bacteria strains, and transformability of these rare metals was investigated by cell-transformation assay using v-Ha-ras-transfected BALB/c 3T3 cells (Bhas 42 cells). Nano-sized Dy2O3 showed strong mutagenesis in all five bacteria strains tested with and without metabolic activation, while micro-sized particles showed weak mutagenesis in two bacterial strains. Dy2O3 induced transformation colonies of Bhas 42 cell dose-dependently, although there was no difference in the number of transformed foci between nano-sized and micro-sized particles. Nano-sized In2O3 and WO3 showed positive mutagenic response in TA1537 and TA98, respectively, whereas the micro-sized metal oxide particles showed no mutagenesis in the test bacterial strains. Both nano-sized and micro-sized In2O3 showed similar levels of transformability. However, nano-sized and micro-sized WO3 did not show any transformability. Both nano-sized and micro-sized Mo particles showed neither mutagenesis nor transformability. These results suggest that mutagenicity of rare metals depends on their particle size, although transformability depends on their chemical components but not on their particle size. Copyright (C) 2011 John Wiley & Sons, Ltd.