Cell fusion induced by herpes simplex virus glycoproteins gB, gD, and gH-gL requires a gD receptor but not necessarily heparan sulfate
Cell fusion induced by herpes simplex virus glycoproteins gB, gD, and gH-gL requires a gD receptor but not necessarily heparan sulfate
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DOI：
10.1006/viro.2000.0713
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发表时间：
2001-01-05
期刊：
影响因子：
3.7
通讯作者：
Spear, PG
中科院分区：
文献类型：
--
作者：
Pertel, PE;Fridberg, A;Spear, PG
为了表征I型单纯疱疹病毒（HSV-1）诱导细胞融合所需的细胞因子，我们使用了一种依赖于转染细胞中HSV-1糖蛋白表达的高效定量分析。我们发现：(1)细胞融合不仅依赖于四种病毒糖蛋白（gB、go和gH-gL）的表达，还依赖于go特异性细胞表面进入受体的表达。(2)细胞融合需要在同一细胞中表达所有四种糖蛋白。(3)细胞融合不需要硫酸希帕兰。[4]受体与这四种糖蛋白在同一细胞中共表达可降低融合活性，表明go与受体的相互作用可限制多核细胞的形成。总的来说，单纯疱疹病毒-1诱导的细胞融合的病毒和细胞决定因素与病毒进入的决定因素相似，除了病毒与细胞表面硫酸肝素结合显著增强了单纯疱疹病毒-1的进入。(C) 2001学术出版社。
To characterize cellular factors required for herpes simplex virus type I (HSV-1)-induced cell fusion, we used an efficient and quantitative assay relying on expression of HSV-I glycoproteins in transfected cells. We showed the following: (1) Cell fusion depended not only on expression of four viral glycoproteins (gB, go, and gH-gL), as previously shown, but also on expression of cell surface entry receptors specific for go. (2) Cell fusion required expression of all four glycoproteins in the same cell. (3) Heparan sulfate was not required for cell fusion. [4) Coexpression of receptor with the four glycoproteins in the same cell reduced fusion activity, indicating that interaction of go and receptor can limit polykaryocyte formation. Overall, the viral and cellular determinants of HsV-1-induced cell fusion are similar to those for viral entry, except that HSV-I entry is significantly enhanced by binding of virus to cell surface heparan sulfate. (C) 2001 Academic Press.