Pharmacological studies on cutaneous inflammation induced by ultraviolet irradiation (1): quantification of erythema by reflectance colorimetry and correlation with cutaneous blood flow.
Pharmacological studies on cutaneous inflammation induced by ultraviolet irradiation (1): quantification of erythema by reflectance colorimetry and correlation with cutaneous blood flow.
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紫外线照射引起的皮肤炎症的药理学研究（1）：反射比色法定量红斑及其与皮肤血流量的相关性。
DOI：
10.1254/jjp.62.245
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
K. Tsurumi
中科院分区：
文献类型：
--
作者：
T. Nose;K. Tsurumi
本研究旨在量化豚鼠紫外线（UV）红斑的强度，这是一种评估抗炎药物的方法，并阐明红斑与皮肤血流量的相关性。用比色仪和激光多普勒血流仪测量紫外线照射后未给药和给药动物的皮肤颜色和皮肤血流量。皮肤颜色由XYZ比色系统和L*a*b*颜色空间表示。在两种比色系统中，随着红斑的加重，x和y或a*和b*两个指标的值均增加。由a*和b*计算的色度（C*）值的增加与紫外线剂量有关。辐照后0.5 ~ 4hr，吲哚美辛可显著抑制该值，是评价药物抑制作用的一个清晰、灵敏的指标。紫外线照射也增加了皮肤血流量。吲哚美辛在紫外线照射2-3小时后显著抑制了这种增加。皮肤血流量变化与C*相关。以上结果提示，C*是量化紫外线红斑的合适参数，紫外线红斑中皮肤颜色的变化反映了皮肤血流量的变化。
This study was conducted to quantify the intensity of ultraviolet (UV) erythema in guinea pigs, a method for evaluating anti-inflammatory drugs, and to clarify any correlation of erythema with cutaneous blood flow. Skin color and cutaneous blood flow in non-administered and indomethacin-administered animals were measured by a colorimeter and a laser Doppler flowmeter over time after UV-irradiation treatment. Skin color was indicated by a XYZ colorimetric system and L*a*b* color space. In either colorimetric system, the values of two indices, x and y or a* and b*, increased along with the intensification of erythema. The increase in the chroma (C*) value calculated from a* and b* was UV-dose-dependent. This value was significantly suppressed by indomethacin 0.5-4 hr after irradiation, and it was found to be a clear and sensitive index for evaluating the suppressive effect of drugs. Cutaneous blood flow also increased with UV irradiation. Indomethacin significantly suppressed this increase 2-3 hr after UV irradiation. The changes of cutaneous blood flow correlated with those of C*. These results suggested C* was a suitable parameter to quantify UV erythema, and the change of skin color in UV erythema reflected the change of cutaneous blood flow.