ISOLATED HETEROCYSTS OF ANABAENA-VARIABILIS SYNTHESIZE ENVELOPE POLYSACCHARIDE
ISOLATED HETEROCYSTS OF ANABAENA-VARIABILIS SYNTHESIZE ENVELOPE POLYSACCHARIDE
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DOI：
10.1016/0304-4165(81)90384-6
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
WOLK, CP
中科院分区：
文献类型：
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作者：
CARDEMIL, L;WOLK, CP
分离的异囊，在30度孵育1小时。用[14C]甘露糖，合成[14C]阿拉伯糖和[14C]葡萄糖，并将3个14C标记的糖以其包膜多糖特有的糖苷键结合到包膜中。在高温80%甲醇的代谢中间体提取物中，[14C]甘露糖与核苷酸GDP相关，[14C]葡萄糖和[14C]阿拉伯糖与UDP相关。杂囊的氯仿/甲醇提取物含有磷酸糖脂，其中含有14c标记的甘露糖、阿拉伯糖和葡萄糖。在不同的色谱条件下，脂质具有与磷酸多醇甘露糖相同的Rf，并且与聚异戊二醇单磷酸糖脂一样，对温和的碱处理稳定，但对温和的酸水解不稳定。如果将杆菌肽添加到培养混合物中，14C标记的核苷酸糖会积累，但14C与包膜多糖的结合会大大减少。聚异戊二醇磷酸糖脂是这种多糖生物合成的中间体。
Isolated heterocysts, incubated for 1 h at 30.degree. C with [14C]mannose, synthesize [14C]arabinose and [14C]glucose, and incorporate the 3 14C-labeled sugars into their envelopes with glycosidic linkages characteristic of their envelope polysaccharide. In extracts of metabolic intermediates with hot 80% methanol, [14C]mannose is associated with the nucleotide GDP and [14C]glucose and [14C]arabinose with UDP. Chloroform/methanolic extracts of the heterocysts contain phosphoglycolipids in which 14C-labeled mannose, arabinose and glucose are present. The lipids have the same Rf as dolichol phosphate mannose under varying chromatographic conditions and like polyisoprenol monophosphate glycolipids are stable to treatment with mild alkali but labile to mild acid hydrolysis. If bacitracin is added to the incubation mixture, 14C-labeled nucleotide sugars accumulate, but incorporation of 14C into envelope polysaccharide is greatly diminished. Polyisoprenol phosphoglycolipids are intermediates in the biosynthesis of this polysaccharide.